Composition for promoting cartilage formation or repair comprising a nell gene product and method of treating cartilage-related conditions using such composition
View Patent ↗Provided herein is a composition for cartilage formation or regeneration comprising a NELL gene product and a method of treating cartilage-related conditions using such a composition.
1. A method of increasing cartilage formation or repair comprising:
applying a NELL-1 peptide or an expression vector encoding said NELL-1 peptide in an effective amount for cartilage formation or repair in a region where cartilage formation or repair is desired;
optionally applying an agent to the region where cartilage formation or repair is desired; and
at least inducing hypertrophy of chondroblast in the region where cartilage formation or repair is desired,
wherein the NELL-1 peptide comprises the amino acid sequence set forth in SEQ ID NO: 2, 4, or 6 or a fragment of the amino acid sequence of SEQ ID NO: 2, 4, or 6 which has cartilage-forming activity.
2. The method of claim 1 , wherein cartilage formation or repair includes bone healing or bone regeneration.
3. The method of claim 1 , wherein the agent is selected from chondroprotective agents, anti-pain and/or anti-inflammatory agents, growth factors, cytokines, small molecules, anti-angiogenic factors, and combinations thereof.
4. The method of claim 1 , wherein the agent is selected from collagen, bone matrix, ligament matrix, tendon matrix, chondrogenic cells, and osteochondroprogenitor cells.
5. A method of treating or ameliorating a cartilage related condition, comprising applying to a site in a mammalian subject a composition comprising a NELL-1 peptide or an expression vector encoding said NELL-1 peptide in an effective amount for cartilage formation or repair;
wherein the NELL-1 peptide comprises the amino acid sequence set forth in SEQ ID NO: 2, 4, or 6 or a fragment of the amino acid sequence of SEQ ID NO: 2, 4, or 6 which has cartilage-forming activity.
6. The method of claim 5 , wherein the composition further comprises a second agent selected from the group consisting of chondroprotective agents, anti-pain and/or anti-inflammatory agents, growth factors, cytokines, small molecules, anti-angiogenic factors and combinations thereof.
7. A method of treating or ameliorating a cartilage related condition, comprising applying to a site in a mammalian subject an implant comprising a substrate having a surface, wherein at least a portion of the surface includes a composition that comprises a NELL-1 peptide or an expression vector encoding said NELL-1 peptide in an effective amount for cartilage formation or repair;
wherein the NELL-1 peptide comprises the amino acid sequence set forth in SEQ ID NO: 2, 4, or 6 or a fragment of the amino acid sequence of SEQ ID NO: 2, 4, or 6 which has cartilage-forming activity.
8. The method of claim 7 , wherein the composition further comprises a second agent selected from the group consisting of chondroprotective agents, anti-pain and/or anti-inflammatory agents, growth factors, cytokines, small molecules, anti-angiogenic factors and combinations thereof.