Nano-porous membrane based sensors
View Patent ↗Sensors include nano-porous alumina membranes that are sensitized by immobilization of antibodies in the nano-pores. The nano-membranes can be sensitized to respond to a single target compound, or different portions of the nano-membrane can be differently sensitized. Capture of the target compound can be detected based on a spectral signature associated with electrical conductance in the nano-pores.
1. A sensor, comprising:
a solid substrate having a top surface;
a nano-porous alumina membrane situated at the top surface of the solid substrate, the alumina membrane including a plurality of nano-pores that do not extend through the alumina membrane, the alumina membrane including a first sensitizing agent for detection of a target compound, the first sensitizing agent being immobilized within a first set of nano-pores so as to define a sensitized portion of the alumina membrane; and
a first conductor electrically coupled to the first sensitizing agent immobilized in the first set of nano-pores defined in the alumina membrane, the first conductor situated on the top surface of the solid substrate and in contact with the sensitized portion of the alumina membrane.
2. The sensor of claim 1 , further comprising:
a second conductor electrically coupled to a second set of nano-pores; and
a second sensitizing agent immobilized within the second set of nano-pores.
3. The sensor of claim 2 , wherein the first sensitizing agent and the second sensitizing agent are the same.
4. The sensor of claim 2 , wherein the first sensitizing agent and the second sensitizing agent are different.
5. The sensor of claim 1 , further comprising a spectrum analyzer in communication with the first conductor, the spectrum analyzer configured to produce an estimate of a received signal portion based on a signature frequency or frequencies associated with specific binding of a target compound to the first sensitizing agent.
6. The sensor of claim 5 , wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with at least two frequencies associated with a detection signature.
7. The sensor of claim 2 , further comprising a spectrum analyzer that is selectively coupled to the first conductor and the second conductor, wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with a frequency or frequencies associated with a first signature and a frequency or frequencies associated with a second signature.
8. The sensor of claim 7 , wherein the spectrum analyzer is configured to produce an estimate of a received signal portion associated with at least two frequencies associated with the first signature and the second signature.
9. An apparatus, comprising:
a base substrate having a plurality of conductor segments defined thereon;
a nano-porous membrane secured to the base substrate, the nano-porous membrane including a plurality of sets of sensitized nano-pores that do not extend through the nano-porous membrane, the nano-pores having respective sensitizers for detection of a target compound, the sensitizers being immobilized within the sets of nano-pores, and the respective sensitizers electrically coupled to corresponding conductor segments; and
a fluid chamber configured to receive a test fluid and communicate the test fluid to the sets of sensitized nano-pores.
10. The apparatus of claim 9 , wherein the nano-porous membrane is an alumina membrane having a plurality of nano-pores with effective diameters in a range of 5 nm to 500 nm.
11. The apparatus of claim 10 , wherein the plurality of sets of nano-pores comprises a first set of nano-pores having a first effective diameter, and a second set of nano-pores having a second effective diameter, and wherein the first effective diameter is larger than the second effective diameter.
12. The apparatus of claim 9 , further comprising a spectrum analyzer configured to receive electrical signals from the conductor segments and produce spectra of the electrical signals.
13. The apparatus of claim 9 , wherein the sets of sensitized nano-pores have associated pore sizes and at least two of the sets are associated with different pore sizes.
14. The sensor of claim 1 wherein the first conductor is a linear conductor.
15. The sensor of claim 1 wherein the first conductor has a shape different than linear.
16. The sensor of claim 2 wherein the first conductor is a linear conductor, or the second conductor is a linear conductor, or both the first and second conductors are linear conductors.
17. The sensor of claim 2 wherein the first conductor has a shape different than linear, or the second conductor has a shape different than linear, or both the first and the second conductors have conductor shapes different than linear.
18. The sensor of claim 1 wherein the sensitizing agent is an antibody.