Method for making β-secretase co-crystals complexed with a ligand
View Patent ↗The present application relates to methods for growing crystals of both the uncomplexed and complexed forms of β-secretase (BACE) polypeptide. The present application also relates to crystalline forms of uncomplexed BACE and the three-dimensional structure of BACE, as determined from the crystals. In addition, the present application relates to the use of crystalline forms of BACE to identify ligands, preferably inhibitors (antagonists), which bind to, and preferably inhibit the enzymatic activity of, BACE. Furthermore, the present application relates to nucleic acid sequences encoding human BACE polypeptide, and methods for making BACE in greater quantity than prior methods, resulting in more effective crystallization.
1. A method for making a crystal comprising a polypeptide complexed to a
OM-99-2 ligand consisting essentially of the steps of:
(a) combining equal volumes of (i) a mixture of 16 mg/ml of polypeptides of SEQ ID NO: 20 and SEQ ID NO: 22 wherein said polypeptides are generated by trans-cleavage processing of a polypeptide of SEQ ID NO: 18; and 1.0 mM of OM-99-2 ligand; with (ii) a crystallization solution, said solution consisting essentially of water, 20% PEG3350 and 0.2 M K + /Na + tartrate; and
(b) crystallizing said combination at about 4° C. using a hanging drop method.