Multi-functional and configurable assay
Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position of the magnet. The assay is multi-configurable and can be modified to isolate target cells and grow cell cultures.
1. A multi-conformable assay system for use in performing a magnetic chromatography method to isolate at least one target cell present in a fluid sample, the assay comprising:
a. a microscope slide having formed thereon a texturized surface portion;
b. a housing positionable upon said microscope slide, said housing having a sample well formed thereon, wherein said sample well is positionable above the texturized surface portion of the microscope slide and wherein the sample well is operative to receive a reaction mixture having a quantity of magnetic particles-antibodies suspended therein, said magnetic particle antibodies being operative to bind with said at least one target cell;
c. at least one absorbent member positionable within said housing and adjacent to said sample well to absorb a portion of said reaction mixture deposited in said sample well not containing said magnetic particles, wherein said texturized surface portion is operative to restrict the flow of said sample flowing from said sample well to said at least one absorbent member; and
d. a magnet positionable below the microscope slide texturized surface portion.
2. The assay of claim 1 wherein said housing comprises a generally block-like structure defining an upper platform surface, said sample well being formed in the general midsection of said upper platform surface, said housing further defining first and second voids on opposed sides of said sample well; said system further comprising first and second absorbent pads, said first and second absorbent pads being received into dedicated ones of said voids of opposed sides of said sample well.
3. The assay of claim 2 wherein said housing is fabricated from a rigid, black material selected from the group consisting of plastic and metal.
4. The assay of claim 1 wherein said texturized surface comprises a plurality of structures uniformly formed upon said texturized surface, said plurality of structures being operative to impede sample flow.
5. The assay of claim 1 wherein said housing is removable from said microscope slide.
6. The assay of claim 3 wherein the housing is fabricated from metal.
7. The assay of claim 4 wherein said texturized surface comprises cylindrical pillars.
8. A multi-conformable assay system for use in performing a magnetic chromatography method to isolate at least one target cell present in a fluid sample, the assay comprising:
a. a cell culture dish having formed thereon a texturized surface portion;
b. a housing positionable upon said cell culture dish, said housing having a sample well formed thereon, wherein said sample well is positionable above the texturized surface portion of the cell culture dish and wherein the sample well is operative to receive a reaction mixture having a quantity of magnetic particles-antibodies suspended therein, said magnetic particle antibodies being operative to bind with said at least one target cell;
c. at least one absorbent member positionable within said housing and adjacent to said sample well to absorb a portion of said reaction mixture deposited in said sample well not containing said magnetic particles, wherein said texturized surface portion is operative to restrict the flow of said sample flowing from said sample well to said at least one absorbent member; and
d. a magnet positionable below the cell culture dish texturized surface portion.
9. The assay of claim 8 wherein said texturized surface comprises a plurality of structures uniformly formed upon said texturized surface, said plurality of structures being operative to impede sample flow.
10. The assay of claim 9 wherein said texturized surface comprises cylindrical pillars.
11. The assay of claim 8 wherein said housing comprises a generally block-like structure defining an upper platform surface, said sample well being formed in the general midsection of said upper platform surface, said housing further defining first and second voids on opposed sides of said sample well; said system further comprising first and second absorbent pads, said first and second absorbent pads being received into dedicated ones of said voids of opposed sides of said sample well.
12. The assay of claim 11 wherein said housing is fabricated from a rigid, black material selected from the group consisting of plastic and metal.
13. The assay of claim 12 wherein the housing is fabricated from metal.
14. The assay of claim 8 wherein said housing is removable from said cell culture dish.