Capillary electrophoresis apparatus and electrophoresis method
An object of the present invention is to provide a capillary electrophoresis apparatus in which simultaneity can be ensured between sensitivity and data acquisition to decrease a pull-up signal while spectral data acquisition is eliminated in each capillary exchange. The invention relates to a capillary electrophoresis apparatus in which capillary position shift is detected in each capillary exchange by detecting a capillary position. A capillary position measuring light source is provided in the capillary electrophoresis apparatus of the invention. The capillary is irradiated with light emitted from the capillary position measuring light source, a capillary image is detected with a two-dimensional detector, and thereby a position deviation of the capillary is determined. On the basis of the position deviation of the capillary, a data acquisition area is set in the two-dimensional detector, or a reference fluorescent light spectrum determined from the capillary at the standard position is corrected.
1. A capillary electrophoresis apparatus comprising:
an exchangeable capillary;
an optical irradiation system including a light source which irradiates the capillary with excitation light;
an optical detection system which has a wavelength dispersion device and a two-dimensional detector, the wavelength dispersion device dispersing fluorescent light from the capillary, the two-dimensional detector detecting a fluorescence spectrum image acquired from the wavelength dispersion device;
a capillary position measuring light source which irradiates the capillary with a capillary position measuring light when the capillary is exchanged; and
a filter including a band pass filter or long-pass filter positioned on the output side of the capillary position measuring light source,
wherein when the capillary is configured to be irradiated with the light from the capillary position measuring light source, the two dimensional detector is configured to: detect a two-dimensional image of the capillary, set a plurality of data acquisition areas based on the position of the image of the capillary, and obtain a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from the plurality of data acquisition areas on the two-dimensional detector.
2. A capillary electrophoresis apparatus according to claim 1 , wherein, when the capillary is shifted in the wavelength dispersive direction with respect to the standard position, the data acquisition areas are moved in the wavelength dispersive direction by a distance corresponding to the position deviation.
3. A capillary electrophoresis apparatus according to claim 1 , wherein, when the capillary is inclined with respect to the standard position, the data acquisition areas are inclined at an angle corresponding to the inclination.
4. A capillary electrophoresis apparatus according to claim 1 , wherein a wavelength scale is set in an image of the two-dimensional detector such that a wavelength of the capillary position measuring light source corresponds to an image position of the capillary by the two-dimensional detector, and a data acquisition area corresponding to a previously given analysis wavelength range is set by reading a long-wavelength edge and a short-wavelength edge of the analysis wavelength range using the wavelength scale.
5. A capillary electrophoresis apparatus comprising:
an exchangeable capillary;
an optical irradiation system including a light source which irradiates the capillary with excitation light;
an optical detection system which has a wavelength dispersion device and a two-dimensional detector, the wavelength dispersion device dispersing fluorescent light from the capillary, the two-dimensional detector detecting a fluorescence spectrum image acquired from the wavelength dispersion device;
a capillary position measuring light source which irradiates the capillary with a capillary position measuring light when the capillary is exchanged; and
a filter including a band pass filter or long-pass filter positioned on the output side of the capillary position measuring light source,
wherein when the capillary is configured to be irradiated with the light from the capillary position measuring light source, the two-dimensional detector is configured to: detect a two-dimensional image of the capillary, obtain a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from a plurality of predetermined data acquisition areas on the two-dimensional detector, and correct a standard position of the fluorescence spectrum image which has been detected when the capillary was position at a standard position based on the position of the image of the capillary.
6. A capillary electrophoresis apparatus according to claim 1 or 5 , wherein the plurality of data acquisition areas are arranged along a capillary axis direction, and each data acquisition area is defined by a range in a wavelength dispersive direction and a range in the capillary axis direction.
7. A capillary electrophoresis apparatus according to claim 1 or 5 , wherein the capillary position measuring light source includes a light source having a single wavelength.
8. A capillary electrophoresis apparatus according to claim 1 or 5 , wherein the capillary position measuring light source has:
a light source which has a predetermined light emission wavelength band.
9. A capillary electrophoresis apparatus according to claim 1 or 5 , wherein the optical irradiation system irradiates the capillary with the excitation light from a direction along an axis line of the capillary or from a direction inclined at a predetermined angle with respect to the axis line of the capillary.
10. A capillary electrophoresis apparatus according to claim 1 or 5 , wherein the optical irradiation system has a light emitting diode which is of an excitation light source.
11. A capillary electrophoresis apparatus according to claim 1 or 5 , wherein the standard position is the capillary position in spectral data acquisition performed before shipment.
12. A capillary electrophoresis method in which a sample is electrophoresed in a capillary, the capillary is irradiated with excitation light emitted from a light source of an optical irradiation system, fluorescent light from the capillary is dispersed to generate a fluorescence spectrum, the fluorescence spectrum is detected by a two-dimensional detector, and the sample is analyzed based on the detection result,
the capillary electrophoresis method including:
irradiating the capillary with a light emitted from a capillary position measuring light source and passed through a filter including a band pass filter or long-pass filter when the capillary is exchanged;
detecting an image of the capillary by the two-dimensional detector;
setting a plurality of data acquisition areas on the two-dimensional detector based on the position of the image of the capillary; and
detecting a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from the plurality of data acquisition areas on the two-dimensional detector.
13. A capillary electrophoresis method in which a sample is electrophoresed in a capillary, the capillary is irradiated with excitation light emitted from a light source of an optical irradiation system, fluorescent light from the capillary is dispersed to generate a fluorescence spectrum, the fluorescence spectrum is detected by a two-dimensional detector, and the sample is analyzed based on the detection result,
the capillary electrophoresis method including:
irradiating the capillary with a light emitted from a capillary position measuring light source and passed through a filter including a band pass filter or long-pass filter when the capillary is exchanged;
detecting an image of the capillary by the two-dimensional detector;
detecting a plurality of fluorescence spectrum images corresponding to a plurality of positions on the capillary from a plurality of predetermined data acquisition areas on the two-dimensional detector; and
correcting a standard position of fluorescence spectrum image which has been detected when the capillary was positioned at the standard position based on the position of the image of the capillary.