Anti-inflammatory dAb
The present invention provides a recombinant domain antibody (dAb) which binds to human TNF-α, the dAb comprising an immunoglobulin heavy or light chain variable domain, wherein the variable domain comprises at least one complementarity determining region (CDR) having a sequence derived from a New World primate.
1. A recombinant domain antibody (dAb) which binds to human TNF-α, wherein the dAb has a sequence selected from the group consisting of:
(SEQ ID No: 8)
DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR;
(SEQ ID No: 9)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR;
(SEQ ID No: 10)
DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR;
(SEQ ID No: 50)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKPPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR;
(SEQ ID No: 51)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGRGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR;
and
(SEQ ID No: 52)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLVPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR.
2. A recombinant dAb according to claim 1 wherein the dAb has the sequence:
(SEQ ID No: 10)
DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR.
3. A pharmaceutical composition comprising an effective amount of a recombinant domain antibody (dAb) according to claim 1 , together with a pharmaceutically acceptable carrier or diluent.
4. A method for detecting human TNF-α in a sample comprising contacting the sample with an effective amount of a recombinant dAb according to claim 1 and detecting the amount of bound dAb.
5. The method according to claim 4 wherein the sample is a biological sample.
6. A recombinant dAb according to claim 1 wherein the dAb has the sequence:
(SEQ ID No: 8)
DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR.
7. A recombinant dAb according to claim 1 wherein the dAb has the sequence:
(SEQ ID No: 9)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR.
8. A recombinant dAb according to claim 1 wherein the dAb has the sequence:
(SEQ ID No: 50)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKPPKLLIYS
ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR.
9. A recombinant dAb according to claim 1 wherein the dAb has the sequence:
(SEQ ID No: 51)
DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS
ASNLETGVPSRFSGRGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ
GTKVEIKR.
10. A recombinant dAb according to claim 1 wherein the dAb has the sequence:
(SEQ ID No: 52)
ADIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIY
SASNLETGVPSRFSGSGSGTDFTLTISSLVPEDFATYYCQQVVWRPFTFG
QGTKVEIKR.