Process for Production of Microbial Fat/Oil Containing Discretional Amount of Diacylglycerol and Said Fat/Oil
In a process for the production of diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid, a process which is characterized in that a microbe being able to produce said fat/oil is incubated and, if desired, said fat/oil is collected.
1 . A process for production of diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid, the process being characterized in that a microbe being able to produce said fat/oil is incubated and, if desired, said fat/oil is collected.
2 . The process according to claim 1 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 30% by weight.
3 . The process according to claim 1 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 40% by weight.
4 . The process according to claim 1 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 50% by weight.
5 . The process according to claim 1 , wherein the microbe is a microbe of genus Mortierella.
6 . The process according to claim 5 , wherein the microbe is a microbe of genus Mortierella , subgenus Mortierella.
7 . The process according to claim 6 , wherein the microbe is a microbe of species Mortierella alpina.
8 . The process according to claim 5 , wherein the microbe is a mutant strain of a microbe which is able to produce fat/oil containing a polyunsaturated fatty acid.
9 . A microbe of genus Mortierella which is able to produce diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is not less than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid.
10 . A microbe of genus Mortierella which is able to produce diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is not less than 30% by weight and a polyunsaturated fatty acid is a constituting fatty acid.
11 . The microbe according to claim 9 , wherein it is Mortierella alpina.
12 . The microbe according to claim 9 , wherein it is a mutant strain.
13 . A microbial cell containing diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid.
14 . The microbial cell according to claim 13 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 30% by weight.
15 . The microbial cell according to claim 13 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 40% by weight.
16 . The microbial cell according to claim 13 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 50% by weight.
17 . The microbial cell according to claim 13 , wherein the microbe is a microbe of genus Mortierella.
18 . The microbial cell according to claim 17 , wherein the microbe is a microbe of species Mortierella alpina.
19 . Fat/oil which is extracted from incubated cells of a microbe being able to produce the fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid.
20 . The fat/oil according to claim 19 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 30% by weight.
21 . The fat/oil according to claim 19 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 40% by weight.
22 . The fat/oil according to claim 19 , wherein the ratio of diacylglycerol to the total neutral lipid is not less than 60% by weight.
23 . The fat/oil according to claim 19 , wherein it is extracted with a nonpolar solvent.
24 . The fat/oil according to claim 19 , wherein it is extracted with a hydrophilic solvent.
25 . A fat/oil in which diacylglycerol and triacylglycerol in a neutral lipid are not less than 70% and not more than 30%, respectively which is prepared in such a manner that fat/oil is extracted from microbial cells containing diacylglycerol and triacylglycerol obtained by incubation of a microbe being able to produce fat/oil where a polyunsaturated fatty acid is a constituting fatty acid and then it is fractionated by distillation.
26 . A fat/oil in which triglyceride and diacylglycerol in a neutral lipid are not less than 95% and not more than 5%, respectively which is prepared in such a manner that fat/oil is extracted from microbial cells containing diacylglycerol and triacylglycerol obtained by incubation of a microbe being able to produce fat/oil where a polyunsaturated fatty acid is a constituting fatty acid and then it is fractionated by distillation.
27 . A fat/oil where the polyunsaturated fatty acid in the fat/oil mentioned in claim 19 is a constituting fatty acid, characterized in that, the polyunsaturated fatty acid constituting the fat/oil is dihomo-γ-linolenic acid (20:3 ω6), arachidonic acid (20:4 ω6), 7,10,13,16-docosatetraenoic acid (22:4 ω6), 4,7,10,13,16-docosapentaenoic acid (22:5 ω6), 6,9,12,15-octadecatetraenoic acid (18:4 ω3), 8,11,14,17-eicosatetraenoic acid (20:4 ω3), eicosapentaenoic acid (20:5 ω3), 7,10,13,16,19-docosapentaenoic acid (22:5 ω3), 4,7,10,13,16,19-docosahexaenoic acid (22:6 ω3), 6,9-octadecadienoic acid (18:2 ω9), 8,11-eicosadienoic acid (20:2 ω9) or 5,8,11-eicosatrienoic acid (mead acid: 20:3 ω9) or a combination thereof.
28 . A food composition, an animal feeds composition, pet food composition, a material for chemical products, a drug and a cosmetic composition in which the fat/oil mentioned in claim 19 is contained, is used as a material or is chemically modified.