Bone tropic peptides
The present invention is directed to the use of bone tropic peptides identified through the use of a phage display library. More particularly, the invention is directed to compositions comprising the bone tropic peptides and methods for using such compositions to regulate osteogenesis, cell adhesion and angiogenesis, and diseases and disorders thereof, and to inhibit cancer cell metastasis and growth.
1. A method of identifying a binding partner of a bone-targeting peptide, wherein said peptide comprises the amino acid sequence selected from the group consisting of SEQ ID NOs:29 and 30, said method comprising contacting said peptide with a subcellular fraction derived from a cell or tissue of interest under conditions to allow binding of said peptide with said binding partner, washing away unbound material from the subcellular fraction, isolating the bound material from said peptide, and characterizing said isolated bound material, thereby identifying a binding partner of a peptide of the invention.
2. The method of claim 1 , wherein said peptide is bound to a solid support.
3. The method of claim 2 , wherein said binding partner is nucleolin or vimentin.
4. An isolated nucleic acid comprising a nucleic acid sequence encoding a peptide comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:29 and 30.
5. A kit for administering a peptide comprising the amino acid sequence selected from the group consisting of SEQ ID NOs:29 and 30, said kit comprising a pharmaceutical composition comprising at least one of said peptides, an applicator, and an instructional material for the use thereof.