IP Library Patent Application 11668976
Patent Application
App. No. 11/668,976

Method of Testing the Safety and Efficacy of a Drug

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Quick Facts
Patent No.
US None
App. No.
11/668,976
Abstract

A method of testing the safety and efficacy of a drug in a culture tray having a plurality of wells surrounded by a chamber wall configured to contain a liquid volume. The method includes depositing different cells of an organism in separate wells of the culture dish, depositing a harmful agent in another of the wells, interconnecting the wells via a fluid medium and introducing a dose of the drug into the fluid medium.

Claims (53)

1 . A method of testing the safety and efficacy of a drug in a culture tray having a plurality of wells surrounded by a chamber wall configured to contain a liquid volume, comprising:

depositing different cells of an organism in separate wells of the culture dish;

depositing a harmful agent in another of the wells;

interconnecting the wells via a fluid medium; and

introducing a dose of the drug into the fluid medium.

2 . The method of claim 1 , further comprising:

determining whether the different cells of the organism are harmed by the dose of the drug.

3 . The method of claim 2 , further comprising:

determining whether growth of the harmful agent is diminished by the dose of the drug.

4 . The method of claim 3 , further comprising:

increasing the dose of the drug if the different cells of the organism are not harmed and the growth of the harmful agent is not diminished.

5 . The method of claim 1 , wherein the harmful agent comprises tumor cells.

6 . The method of claim 5 , wherein the drug comprises an anti-tumor medication.

7 . The method of claim 1 , wherein the different cells of the organism comprise liver, kidney, heart, lung, spleen, and brain cells of the human body.

8 . The method of claim 1 , further comprising:

increasing the dose of the drug until the drug harms the different cells of the organism; and

designating the dose of the drug at which the different cells of the organism are harmed as a toxic dose level.

9 . The method of claim 1 , further comprising:

increasing the dose of the drug until the effect of the harmful agent is reduced; and

designating the dose of the drug at which the effect of the harmful agent is reduced as an effective dose level.

10 . The method of claim 1 , wherein:

the harmful agent comprises cholesterol;

the drug comprises an anti-cholesterol drug; and

the different cells include liver cells.

11 . The method of claim 1 , wherein:

the harmful agent comprises cancer cells; and

the drug includes an undesirable toxicity above a certain dose.

12 . The method of claim 1 , further comprising:

depositing one or more culture medium in the separate wells.

13 . The method of claim 1 , further comprising:

culturing the different cells in the separate wells.

14 . A method of simultaneously conducting multiple tests of the safety and efficacy of a drug in a culture tray having more than one set of wells, each set of wells being segregated in a chamber, comprising:

depositing different cells of an organism in separate wells in each chamber;

depositing a harmful agent in one of the wells in each chamber;

interconnecting the wells in each chamber via a fluid medium; and

introducing a dose of the drug into the fluid medium in each chamber with the dose of the drug being varied in each chamber.

15 . The method of claim 14 , further comprising:

providing the tray segregated into 12 chamber units each having a chamber wall surrounding 8 of the wells.

16 . The method of claim 14 , further comprising:

providing the tray segregated into 64 chamber units each having a chamber wall surrounding 6 of the wells.

17 . The method of claim 14 , further comprising:

providing the tray segregated into 256 chamber units each having a chamber wall surrounding 6 of the wells.

18 . The method of claim 14 , further comprising:

depositing a one or more culture medium in the separate wells in each chamber.

19 . The method of claim 14 , further comprising:

culturing the different cells in the separate wells.

20 . A method of testing the safety and efficacy of a drug in a culture tray having a plurality of wells surrounded by a chamber wall configured to contain a liquid volume, comprising:

depositing a first cell type in a first well and a second cell type in a second well;

depositing a harmful agent in a third well;

interconnecting the first, second and third well via a fluid medium contained by the chamber wall;

introducing a dose of the drug into the fluid medium;

determining whether the first cell type or the second cell type are harmed by the dose of the drug; and

determining whether growth of the harmful agent is inhibited by the dose of the drug.

Assignments (2)
MERGER Recorded Jan 25, 2023
From: ADVANCED PHARMACEUTICAL SCIENCES, LLC
To: DISCOVERY LIFE SCIENCES, LLC
Reel/Frame 062480/0699 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 3, 2007
From: LI, ALBERT P.
To: ADVANCED PHARMACEUTICAL SCIENCES, INC.
Reel/Frame 018849/0500 →