Method of Testing the Safety and Efficacy of a Drug
A method of testing the safety and efficacy of a drug in a culture tray having a plurality of wells surrounded by a chamber wall configured to contain a liquid volume. The method includes depositing different cells of an organism in separate wells of the culture dish, depositing a harmful agent in another of the wells, interconnecting the wells via a fluid medium and introducing a dose of the drug into the fluid medium.
1 . A method of testing the safety and efficacy of a drug in a culture tray having a plurality of wells surrounded by a chamber wall configured to contain a liquid volume, comprising:
depositing different cells of an organism in separate wells of the culture dish;
depositing a harmful agent in another of the wells;
interconnecting the wells via a fluid medium; and
introducing a dose of the drug into the fluid medium.
2 . The method of claim 1 , further comprising:
determining whether the different cells of the organism are harmed by the dose of the drug.
3 . The method of claim 2 , further comprising:
determining whether growth of the harmful agent is diminished by the dose of the drug.
4 . The method of claim 3 , further comprising:
increasing the dose of the drug if the different cells of the organism are not harmed and the growth of the harmful agent is not diminished.
5 . The method of claim 1 , wherein the harmful agent comprises tumor cells.
6 . The method of claim 5 , wherein the drug comprises an anti-tumor medication.
7 . The method of claim 1 , wherein the different cells of the organism comprise liver, kidney, heart, lung, spleen, and brain cells of the human body.
8 . The method of claim 1 , further comprising:
increasing the dose of the drug until the drug harms the different cells of the organism; and
designating the dose of the drug at which the different cells of the organism are harmed as a toxic dose level.
9 . The method of claim 1 , further comprising:
increasing the dose of the drug until the effect of the harmful agent is reduced; and
designating the dose of the drug at which the effect of the harmful agent is reduced as an effective dose level.
10 . The method of claim 1 , wherein:
the harmful agent comprises cholesterol;
the drug comprises an anti-cholesterol drug; and
the different cells include liver cells.
11 . The method of claim 1 , wherein:
the harmful agent comprises cancer cells; and
the drug includes an undesirable toxicity above a certain dose.
12 . The method of claim 1 , further comprising:
depositing one or more culture medium in the separate wells.
13 . The method of claim 1 , further comprising:
culturing the different cells in the separate wells.
14 . A method of simultaneously conducting multiple tests of the safety and efficacy of a drug in a culture tray having more than one set of wells, each set of wells being segregated in a chamber, comprising:
depositing different cells of an organism in separate wells in each chamber;
depositing a harmful agent in one of the wells in each chamber;
interconnecting the wells in each chamber via a fluid medium; and
introducing a dose of the drug into the fluid medium in each chamber with the dose of the drug being varied in each chamber.
15 . The method of claim 14 , further comprising:
providing the tray segregated into 12 chamber units each having a chamber wall surrounding 8 of the wells.
16 . The method of claim 14 , further comprising:
providing the tray segregated into 64 chamber units each having a chamber wall surrounding 6 of the wells.
17 . The method of claim 14 , further comprising:
providing the tray segregated into 256 chamber units each having a chamber wall surrounding 6 of the wells.
18 . The method of claim 14 , further comprising:
depositing a one or more culture medium in the separate wells in each chamber.
19 . The method of claim 14 , further comprising:
culturing the different cells in the separate wells.
20 . A method of testing the safety and efficacy of a drug in a culture tray having a plurality of wells surrounded by a chamber wall configured to contain a liquid volume, comprising:
depositing a first cell type in a first well and a second cell type in a second well;
depositing a harmful agent in a third well;
interconnecting the first, second and third well via a fluid medium contained by the chamber wall;
introducing a dose of the drug into the fluid medium;
determining whether the first cell type or the second cell type are harmed by the dose of the drug; and
determining whether growth of the harmful agent is inhibited by the dose of the drug.