Method to Obtain Purified Human Genomic Nucleic Acid
The present invention provides a method to rapidly provide genotype screening of a plurality of biological samples in a designated well of a microwell container for remote user by a screening laboratory. The screening method can be used to determine if a biological sample is heterozygous, homozygous or wild for a designated genetic sequence.
1 . A method to obtain purified human genomic nucleic acid from a sample using magnetic particles comprising:
(a) treating said sample to obtain a lysate containing cellular debris including at least a portion of intact genomic nucleic acid;
(b) treating said lysate to bind said genomic nucleic acid to said magnetic particles;
(c) separating said genomic nucleic acid using magnetic particles; and
(d) disassociating said genomic nucleic acid from said magnetic particles to obtain said purified human genomic nucleic acid including at least a portion of intact genomic nucleic acid.
2 . A method of claim 1 wherein the sample is tissue.