NASAL DECOLONIZATION OF MICROBES
The present invention presents devices, kits, systems and methods that can be used for nasal decolonization of microbes and treatment of MRSA, E. coli, and E. fecalis. For example, it includes a method for nasal decolonization of microbes comprising: applying a photosensitizing composition comprising methylene blue to anterior nares; inserting at least a portion of a light delivery device into the nasal cavity; and applying light to the anterior nares at a wavelength ranges from about 650 nm to 680 nm; wherein the light is delivered by the light delivery device to the anterior nares; the light delivery device is in light communication with a light source via a waveguide; and over 90% of the microbes at the anterior nares is eliminated by the nasal decolonization.
1 . A method for nasal decolonization of microbes comprising:
(a) applying a photosensitizing composition comprising methylene blue to anterior nares;
(b) inserting at least a portion of a light delivery device into the nasal cavity; and
(c) applying light to the anterior nares at a wavelength ranges from about 650 nm to 680 nm; wherein the light is delivered by the light delivery device to the anterior nares without causing physiological damage to host tissue within the nasal cavity; the light delivery device is in light communication with a light source via a waveguide; and over 90% of the microbes at the anterior nares is eliminated by the nasal decolonization.
2 . The method of claim 1 wherein the light application step is repeated for at least one more time.
3 . The method of claim 1 wherein the light application step is repeated for about 2 more times to about four more times.
4 . The method of claim 1 wherein light energy provided by each of the light application step to the treatment site ranges from about 5 J/cm 2 to about 20 J/cm 2 .
5 . The method of claim 1 wherein light energy provided by each of the light application step to the treatment site ranges from about 6 J/cm 2 to about 12 J/cm 2 .
6 . The method of claim 1 wherein step (a) to (c) were repeated as necessary to further eliminate the microbes at the anterior nares.
7 . The method of claim 1 wherein the methylene blue at a concentration ranges from about 0.001 wt % to about 0.1 wt %.
8 . The method of claim 1 wherein the time required for each of the light application step ranges from about 15 seconds to 90 seconds.
9 . The method of claim 1 wherein the wavelength ranges from about 665 nm to about 675 nm.
10 . The method of claim 1 wherein the photosensitizing composition is placed in contact with the anterior nares for about 10 seconds prior to the application of light.
11 . The method of claim 1 wherein the microbes are selected from the group consisting of staphylococcus aureus, MRSA and a combination thereof.
12 . The method of claim 1 wherein the photosensitizing composition further comprises a therapeutic agent.
13 . The method of claim 1 wherein the photosensitizing composition further comprises a carrier for methylene blue.
14 . The method of claim 1 wherein the photosensitizing composition is in a phase selected from the group consisting of liquid, gel, paste, putty, and solid.
15 . The method of claim 1 wherein the light delivery device is an optical probe, the waveguide is an optical fiber, and the light source is a laser.
16 . A method for treatment of MRSA comprising: applying a photosensitizing composition comprising methylene blue to the treatment site where the MRSA is located; applying light to the treatment site at a wavelength ranges from about 650 nm to 680 nm; wherein the light is delivered by the light delivery device to the treatment site; the light delivery device is in light communication with a light source via a waveguide; and over 90% of the MRSA at the treatment site is eliminated by the treatment method.
17 . The method of claim 16 wherein the light application step does not cause physiological damage to host tissue at or around the treatment site.
18 . The method of claim 16 wherein the light application step is repeated for at least one more time.
19 . The method of claim 16 wherein light energy provided by each of the light application step to the treatment site ranges from about 5 J/cm 2 to about 20 J/cm 2 .
20 . The method of claim 16 wherein light energy provided by each of the light application step to the treatment site ranges from about 6 J/cm 2 to about 12 J/cm 2 .
21 . The method of claim 16 wherein light energy provided by each of the light application step to the treatment site ranges from about 6 J/cm 2 to about 12 J/cm 2 and the treatment time required for each of the light application step ranges from about 15 seconds to 90 seconds.
22 . The method of claim 16 wherein all steps of the method were repeated as necessary to further eliminate the microbes at the treatment site.
23 . The method of claim 16 wherein the methylene blue is at a concentration ranges from about 0.02 wt % to about 0.1 wt %.
24 . The method of claim 16 wherein the photosensitizing composition is placed in contact with the treatment site for about 10 seconds prior to the application of light.
25 . The method of claim 16 wherein the light delivery device is an optical probe, the waveguide is an optical fiber, and the light source is a laser.
26 . A method for treatment of E. coli comprising: applying a photosensitizing composition comprising methylene blue to the treatment site where the E. coli is located; applying light to the treatment site at a wavelength ranges from about 650 nm to 680 nm; wherein the light is delivered by the light delivery device to the treatment site; the light delivery device is in light communication with a light source via a waveguide; and over 90% of the E. coli at the treatment site is eliminated by the treatment method.
27 . The method of claim 26 wherein the light application step does not cause physiological damage to host tissue at or around the treatment site.
28 . The method of claim 26 wherein the light application step is repeated for at least one more time.
29 . The method of claim 26 wherein light energy provided by each of the light application step to the treatment site ranges from about 6 J/cm 2 to about 12 J/cm 2 .
30 . The method of claim 26 wherein all steps were repeated as necessary to further eliminate the microbes at the treatment site.
31 . The method of claim 26 wherein the methylene blue is at a concentration ranges from about 0.02 wt % to about 0.1 wt %.
32 . The method of claim 26 wherein the photosensitizing composition is placed in contact with the treatment site for about 10 seconds prior to the application of light.
33 . The method of claim 26 wherein the light delivery device is an optical probe, the waveguide is an optical fiber, and the light source is a laser.
34 . A method for treatment of E. fecalis comprising: applying a photosensitizing composition comprising methylene blue to the treatment site where the E. fecalis is located; applying light to the treatment site at a wavelength ranges from about 650 nm to 680 nm; wherein the light is delivered by the light delivery device to the treatment site; the light delivery device is in light communication with a light source via a waveguide; and over 90% of the E. fecalis at the treatment site is eliminated by the treatment method.
35 . The method of claim 34 wherein the light application step does not cause physiological damage to host tissue at or around the treatment site.
36 . The method of claim 34 wherein the light application step is repeated at least one more time.
37 . The method of claim 34 wherein light energy provided by each of the light application step to the treatment site ranges from about 6 J/cm 2 to about 12 J/cm 2 .
38 . The method of claim 34 wherein all steps were repeated as necessary to further eliminate the microbes at the treatment site.
39 . The method of claim 34 wherein the methylene blue is at a concentration ranges from about 0.02 wt % to about 0.1 wt %.
40 . The method of claim 34 wherein the photosensitizing composition is placed in contact with the treatment site for about 10 seconds prior to the application of light.
41 . The method of claim 34 wherein the light delivery device is an optical probe, the waveguide is an optical fiber, and the light source is a laser.