T1R taste receptors and genes encoding same
Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular taste stimulus in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.
1. A vector containing an isolated nucleic acid which encodes a human hT1R2 taste receptor, wherein said hT1R2 encoding nucleic acid is selected from the following:
(i) a nucleic acid which encodes the human T1R2 polypeptide in SEQ ID NO: 21;
(ii) a nucleic acid which encodes a polypeptide having at least 90% sequence identity to the human T1R2 polypeptide in SEQ ID NO: 21;
(iii) a nucleic acid which hybridizes to the complement of the human T1R2 encoding nucleic acid sequence contained in SEQ ID NO: 23 under stringent hybridization conditions which consist of hybridization in 50% formamide, 5×SSC and 1% SDS, incubating at 42° C., with wash in 0.2×SSC and 0.1% SDS at 65° C., wherein said hybridization and wash steps are each carried out for at least 1 minute; and
(iv) a nucleic acid encoding a human T1R2 polypeptide having the sequence in SEQ ID NO: 21.
2. A recombinant host cell containing a vector according to claim 1 .
3. A method of using the recombinant host cell of claim 2 to express a human T1R2 polypeptide encoded by said vector comprising culturing the host cell under conditions that result in the expression of said human T1R2 polypeptide.
4. The method of claim 3 wherein the cell is a eukaryotic host cell.
5. The method of claim 4 wherein the eukaryotic cell is a mammalian cell.
6. The method of claim 5 wherein the mammalian cell is selected from a CHO cell, COS cell , HEK cell and a BHK cell.
7. The method of claim 4 wherein the cell expresses a G protein that functionally couples with said T1R2 receptor.
8. The method of claim 3 wherein the human T1R2 nucleic acid encodes the human T1R2 polypeptide having SEQ ID NO: 21.
9. The method of claim 3 wherein the human T1R2 nucleic acid encodes a human T1R2 polypeptide which possesses at least 90% sequence identity to the entire human T1R2 polypeptide in SEQ. ID NO: 21.
10. The method of claim 9 wherein the human T1R2 nucleic acid encodes a T1R2 polypeptide having at least 95% sequence identity to the entire polypeptide in SEQ ID NO:21.