Glycoprotein synthesis and remodeling by enzymatic transglycosylation
View Patent ↗A chemoenzymatic method for the preparation of a homogeneous glycoprotein or glycopeptide, including (a) providing an acceptor selected from the group consisting of GlcNAc-protein and GlcNAc-peptide; and (b) reacting the acceptor with a donor substrate including an activated oligosaccharide moiety, in the presence of a catalyst comprising endoglycosidase (ENGase), to transfer the oligosaccharide moiety to the acceptor and yield the homogeneous glycoprotein or glycopeptide. The donor substrate includes, in a specific implementation, a synthetic oligosaccharide oxazoline. A related method of glycoprotein or glycopeptide remodeling with a predetermined natural N-glycan or a tailor-made oligosaccharide moiety, and a method of remodeling an antibody including a heterogeneous sugar chain, are also described. The disclosed methodology enables glycoprotein drugs to be modified for prolonged half-life in vivo, reduced immunogenicity, and enhanced in vivo activity, and for targeting and drug delivery.
1. A method of generating remodeled and homogeneous functionalized glycopeptide or glycoprotein, the method comprising:
(a) preparing an acceptor GlcNAc-containing peptide or protein that accepts an oligosaccharide; and
(b) enzymatically adding the oligosaccharide to the acceptor GlcNAc-containing peptide or protein catalyzed by an endoglycosidase enzyme selected from the group consisting of Endo A and Endo M, wherein the oligosaccharide is a synthetic oligosaccharide oxazoline comprising a sugar chain containing monosaccharide residues and glycosidic linkages to form an intermediate glycopeptide or glycoprotein; and
(c) adding a biologically active agent or a tag to the intermediate glycopeptide or glycoprotein to form the functionalized glycopeptide or glycoprotein.
2. The method of claim 1 , wherein the acceptor GlcNAc-containing peptide or protein is an antibody or fragment thereof.
3. The method of claim 1 , wherein the synthetic oligosaccharide oxazoline is a di-, tri-, tetra-, penta-, hexyl-, hepta-, octyl-, nona-, deca-, or undeca-saccharide oxazoline.
4. The method of claim 1 , wherein the intermediate glycopeptide or glycoprotein further comprises an azido group; wherein the biologically active agent or tag further comprises an alkynyl group; and wherein in step (c) the adding comprises reacting said azido group and said alkynyl group to form a triazole linker group in said functionalized glycopeptide or glycoprotein.
5. The method of claim 1 , wherein the step of preparing an acceptor GlcNAc-containing peptide or protein comprises
(a) providing a peptide or protein substrate comprising at least two GlcNAc residues and at least one GlcNAc-GlcNAc bond; and
(b) treating the peptide or protein substrate with an endoglycosidase to hydrolyze a bond between two of the GlcNAc residues positioned closest to the peptide or protein substrate thereby forming a peptide or protein substrate with a single GlcNAc-moiety.
6. The method of claim 1 , wherein the tag is a drug, toxin, fluorescent probe, biotin, a PEG, lipid, or polypeptide.
7. The method of claim 6 , wherein the tag is polypeptide selected from the group consisting of adrenocorticotropic hormone (ACTH); ebiratide; angiotensin; angiotensin II; asparaginase; atrial natriuretic peptide; atrial sodium diuretic peptide; bacitracin; beta-endorphin; blood coagulation factors VII, VIII and IX; blood thymic factor (FTS); blood thymic factor derivatives; bombesin; bone morphogenic factor; bone morphogenic protein (BMP); bradykinin; caerulein; calcitonin gene related polypeptide (CGRP); calcitonins; a cell growth factor selected from the group consisting of EGF, TGF-alpha, TGF-beta, PDGF, acidic FGF, and basic FGF; cerulein; chemokine; cholecystokinin; cholecystokinin-8 (CCK-8); cholecystokinin-pancreozymin (CCK-PZ); colistin; colony-stimulating factor; corticotropin-releasing factor (CRF); cytokine; desmopressin; dinorphin; a dipeptide; dismutase; dynorphin; eledoisin; endorphin; endothelin; endotherin; enkephalin; epidermal growth factor (EGF); erythropoietin (EPO); follicle-stimulating hormone (FSH); gallanin; gastric inhibitory polypeptide; gastrin-releasing polypeptide (GRP); gastrin; G-CSF; glucagon; glutathione peroxidase; glutathio-peroxidase; a gonadotropin selected from the group consisting of human chorionic gonadotrophin (HCG) and .alpha. and .beta. subunits thereof; gramicidin; gramicidines; growth factor (EGF); growth hormone-releasing factor (GRF); growth hormone; human artrial natriuretic polypeptide (h-ANP); human placental lactogen; insulin; insulin-like growth factor; interferon; interleukin; kallikrein; kyotorphin; luliberin; luteinizing hormone (LH); luteinizing hormone-releasing hormone (LH-RH); lysozyme chloride; melanocyte-stimulating hormone (MSH); melanophore stimulating hormone; mellitin; motilin; muramyl; muramyldipeptide; nerve growth factor (NGF); neuropeptide Y; neurotensin; oxytocin; pancreastatin; pancreatic polypeptide; pancreozymin; parathyroid hormone (PTH); pentagastrin; pituitary adenyl cyclase-activating polypeptides (PACAPs); platelet-derived growth factor (PDGF); polymixin B; prolactin; protein synthesis stimulating polypeptide; PTH-related protein; relaxin; renin; secretin; serum thymic factor; somatomedins; thrombopoietin (TPO); thymic humoral factor (THF); thymopoietin; thymosin; thymostimulin; thyroid hormone releasing hormone; thyroid-stimulating hormone (TSH); thyrotropin releasing hormone (TRH); trypsin; tuftsin; tumor growth factor (TGF-alpha); tumor necrosis factor (TNF); tyrocidin; urogastrone; urokinase; vasoactive intestinal polypeptide (VIP); and vasopressin.
8. The method of claim 6 , wherein the tag is a drug.
9. The method of claim 2 , wherein the GlcNAc containing peptide or protein is an antibody selected from the group consisting of 17b, 48d, A32, C11, 2G12, F240, IgG1b12, 19e, X5, TNX-355 and F91.