Tissue embedding matrix
View Patent ↗The invention is a matrix for preparing a tissue sample made of saline, albumin, gelatin, formaldehyde, a detergent, sucrose, lysine, and glutaraldehyde. The matrix is formed by first preparing a solution of saline, albumin, and gelatin, and then mixing the solution with formaldehyde, a detergent, and sucrose. Immediately prior to fixation of the tissue, a separately prepared lysine-glutaraldehyde mixture is added. The resulting matrix will harden rapidly and may be used to fix tissue samples for histological processing. In particular, a layer of the matrix may be poured into a mold and allowed to harden to form a base. One or more tissue samples may then be pinned to the base of matrix in the mold and then covered with the matrix. Once the matrix hardens, the pins and mold may be removed, and the tissue sample or samples embedded in the matrix are ready for sectioning.
1. A matrix for preparing a tissue sample, comprising:
between about 14.3 percent and 14.5 percent weight by volume of egg albumin;
about 0.2 percent weight by volume of gelatin;
about 0.4 percent weight by volume of NaCl;
about 2.3 percent weight by volume of formaldehyde;
about 0.6 percent weight by volume of a non-ionic detergent;
between about 30.8 percent and about 31.4 percent weight by volume of sucrose;
between about 1.1 percent and 1.5 percent weight by volume of lysine; and
between about 1.2 percent and 1.6 percent weight by volume of glutaraldehyde.
2. The matrix of claim 1 , wherein said lysine is L-lysine mono hydrochloride.
3. The matrix of claim 1 , wherein said non-ionic detergent is polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether