Methods of increasing secretion of polypeptides having biological activity
The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.
1. A method for producing a secreted polypeptide having biological activity, comprising:
(a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, wherein the fusion protein construct comprises:
(i) a first polynucleotide comprising a nucleotide sequence encoding a signal peptide;
(ii) a second polynucleotide comprising a nucleotide sequence encoding at least a catalytic domain of a Humicola Cel45 endoglucanase; and
(iii) a third polynucleotide comprising a nucleotide sequence encoding at least a catalytic domain of a polypeptide having biological activity;
wherein the signal peptide and at least the catalytic domain of the Humicola Cel45 endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the Humicola Cel45 endoglucanase;
(b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and
(c) recovering the fusion protein comprising the amino acid sequence of SEQ ID NO: 74 or SEQ ID NO: 76 from the cultivation medium, wherein the fusion protein has biological activity.
2. The method of claim 1 , wherein the fusion protein construct further comprises a polynucleotide encoding a carbohydrate binding module.
3. The method of claim 1 , wherein the fusion protein construct further comprises (1) a polynucleotide encoding a linker located between the nucleotide sequence encoding at least the Humicola Cel45 endoglucanase catalytic domain and the nucleotide sequence encoding at least the catalytic domain of a polypeptide having biological activity; (2) a polynucleotide encoding a cleavage site located between the nucleotide sequence encoding at least the Humicola Cel45 endoglucanase catalytic domain and the nucleotide sequence encoding at least the catalytic domain of a polypeptide having biological activity; or (3) a combination of (1) and (2).
4. The method of claim 1 , wherein the fusion protein construct further comprises a fourth polynucleotide encoding a second signal peptide.
5. The method of claim 1 , wherein the fusion protein has cellulolytic activity.
6. The method of claim 1 , wherein the polypeptide having biological activity is a beta-glucosidase.
7. The method of claim 1 , wherein the construct further comprises a promoter and a terminator.
8. The method of claim 7 , wherein the promoter drives expression of the first, second, and third polynucleotides, and a fourth polynucleotide encoding a second signal peptide.
9. The method of claim 1 , wherein the recovered polypeptide having biological activity is a polypeptide having beta-glucosidase activity selected from the group consisting of a beta-glucosidase fusion protein, components of the beta-glucosidase fusion protein, and a combination of beta-glucosidase fusion protein and the components thereof.