Nucleic acid and amino acid sequences relating to
View Patent ↗The invention provides isolated polypeptide and nucleic acid sequences derived from Streptococcus pneumoniae that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.
1. An isolated nucleic acid encoding a S. pneumoniae polypeptide comprising SEQ ID NO: 5326.
2. A recombinant expression vector comprising the nucleic acid of claim 1 operably linked to a transcription regulatory element.
3. A cell comprising the recombinant expression vector of claim 2 .
4. An isolated nucleic acid sequence selected from the group consisting of:
a) a nucleic acid encoding a S. pneumoniae polypeptide comprising SEQ ID NO: 5326;
b) a nucleic acid sequence fully complementary to a nucleic acid encoding a polypeptide comprising SEQ ID NO: 5326; and
c) an RNA of a) or b), wherein U is substituted for T.
5. A recombinant expression vector comprising the nucleic acid of claim 4 operably linked to a transcription regulatory element.
6. A cell comprising the recombinant expression vector of claim 5 .
7. An isolated nucleic acid encoding a S. pneumoniae polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO: 5326, wherein the polypeptide elicits an immune response against S. pneumoniae.
8. A recombinant expression vector comprising the nucleic acid of claim 7 operably linked to a transcription regulatory element.
9. A cell comprising the recombinant expression vector of claim 8 .
10. An isolated nucleic acid selected from the group consisting of:
a) a nucleic acid encoding a S. pneumoniae polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO: 5326;
b) a nucleic acid fully complementary to a nucleic acid encoding a polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO: 5326; and
c) an RNA of a) or b), wherein U is substituted for T,
wherein the polypeptide elicits an immune response against S. pneumoniae.
11. A recombinant expression vector comprising the nucleic acid of claim 10 operably linked to a transcription regulatory element.
12. A cell comprising the recombinant expression vector of claim 11 .
13. An isolated nucleic acid encoding a S. pneumoniae polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO: 5326, wherein the polypeptide provides immunity against S. pneumoniae infection.
14. A recombinant expression vector comprising the nucleic acid of claim 13 operably linked to a transcription regulatory element.
15. A cell comprising the recombinant expression vector of claim 14 .
16. An isolated nucleic acid selected from the group consisting of:
a) a nucleic acid encoding a S. pneumoniae polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO: 5326;
b) a nucleic acid fully complementary to a nucleic acid encoding a polypeptide having at least 80% identity to the amino acid sequence of SEQ ID NO: 5326; and
c) an RNA of a) or b), wherein U is substituted for T,
wherein the polypeptide provides immunity against S. pneumoniae infection.
17. A recombinant expression vector comprising the nucleic acid of claim 16 operably linked to a transcription regulatory element.
18. A cell comprising the recombinant expression vector of claim 17 .
19. The isolated nucleic acid of claim 7 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 90% identity to SEQ ID NO: 5326.
20. The isolated nucleic acid of claim 7 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 95% identity to SEQ ID NO: 5326.
21. The isolated nucleic acid of claim 7 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 98% identity to SEQ ID NO: 5326.
22. The isolated nucleic acid of claim 7 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 99% identity to SEQ ID NO: 5326.
23. The isolated nucleic acid of claim 13 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 90% identity to SEQ ID NO: 5326.
24. The isolated nucleic acid of claim 13 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 95% identity to SEQ ID NO: 5326.
25. The isolated nucleic acid of claim 13 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 98% identity to SEQ ID NO: 5326.
26. The isolated nucleic acid of claim 13 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 99% identity to SEQ ID NO: 5326.
27. The isolated nucleic acid of claim 10 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 90% identity to SEQ ID NO: 5326.
28. The isolated nucleic acid of claim 10 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 95% identity to SEQ ID NO: 5326.
29. The isolated nucleic acid of claim 10 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 98% identity to SEQ ID NO: 5326.
30. The isolated nucleic acid of claim 10 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 99% identity to SEQ ID NO: 5326.
31. The isolated nucleic acid of claim 16 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 90% identity to SEQ ID NO: 5326.
32. The isolated nucleic acid of claim 16 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 95% identity to SEQ ID NO: 5326.
33. The isolated nucleic acid of claim 16 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 98% identity to SEQ ID NO: 5326.
34. The isolated nucleic acid of claim 16 , wherein the nucleic acid encodes a S. pneumonaie polypeptide having at least 99% identity to SEQ ID NO: 5326.
35. The isolated nucleic acid of claim 7 , wherein the polypeptide is a surface protein.
36. The isolated nucleic acid of claim 10 , wherein the polypeptide is a surface protein.
37. The isolated nucleic acid of claim 13 , wherein the polypeptide is a surface protein.
38. The isolated nucleic acid of claim 16 , wherein the polypeptide is a surface protein.