Method and apparatus for imaging in an eye

1. A method for in vivo imaging a retinal cell, the method comprising:

obtaining a first, in-vivo image of the at least one retinal cell;

obtaining a second, in-vivo image of the at least one retinal cell;

adjusting at least one of the first, in-vivo image and the second, in-vivo image to account for a movement of the at least one retinal cell; and

generating a final, in-vivo image based at least in part on the adjusting.

2. The method of claim 1 , further comprising obtaining the first image and the second image simultaneously or substantially simultaneously.

3. The method of claim 1 , wherein the first image is captured with a first detector and the second image is captured with a second detector.

4. The method of claim 1 , wherein the first image is captured at a first frequency and the second image is captured at a second frequency.

5. The method of claim 1 , wherein the generating step further comprises averaging the first image and the second image.

6. The method of claim 1 , further comprising:

providing at least one dye to the at least one cell; and

optically exciting the at least one dye.

7. The method of claim 6 , wherein the at least one dye includes at least one of rhodamine, Alexa 594, green fluorescence protein, and fluorescein.

8. The method of claim 6 , wherein the providing includes injecting the at least one dye into at least one layer of a lateral geniculate nucleus (LGN).

9. The method of claim 1 , wherein the cell includes at least one of a retinal ganglion, a retinal pigment epithelial cell, a capillary cell, and a Muller cell.

10. The method of claim 6 , wherein the exciting includes exciting the at least one dye using at least one of a 530 nm light, a 568 nm light, 830 nm light, and a 904 nm light.

11. The method according to claim 6 , wherein the obtaining includes obtaining the at least one image using an adaptive optics scanning laser ophthalmoscope (AOSLO).

12. The method according to claim 11 , wherein the AOSLO includes at least two imaging lasers and at least two detectors.

13. The method according to claim 6 , wherein the obtaining includes obtaining at least two images from at least two spectral bands simultaneously or approximately simultaneously.

14. The method of claim 1 , wherein the first image includes at least one of a reflectance image and a fluorescence image.

15. The method of claim 14 , wherein the second image includes at least one of a reflectance image and a fluorescence image.

16. The method of claim 1 , wherein the second image includes at least one of a reflectance image and a fluorescence image.

17. The method of claim 1 , further comprising:

obtaining a reflectance image of the at least one cell;

obtaining a fluorescence image of the at least one cell;

adjusting at least one of the reflectance image and the fluorescence image to account for a movement of the at least one cell; and

generating the final, in-vivo image based at least in part on the adjusting.

18. The method of claim 17 , further comprising removing warp from at least one of the at least two images.

19. The method of claim 18 , wherein the removing includes:

measuring horizontal warp using a linear line-pair grating positioned at a focal plane of a model eye lens inserted at a pupil plane of the eye; and

performing at least one of stretching and compressing the at least one of the at least two images to correct the warp.

20. The method of claim 17 , wherein the at least one cell includes at least one blood vessel.