Separating agent for optical isomers and separation column for optical isomers
This invention provides a separating agent for optical isomers, which has high asymmetry recognition ability and can be used particularly at a high flow rate when used for the separation of the optical isomers, and a separation column for optical isomers having the same. This invention provides: a separating agent for optical isomers which is used for separation of optical isomers in a sample comprising the optical isomers, which is comprising a monolithic inorganic type carrier having a meso pore formed on an inner wall surface of a specific macropore, and a polysaccharide or a polysaccharide derivative supported on the monolithic inorganic type carrier, wherein the meso pore has a pore size of 6 to 100 nm; and a separation column for optical isomers in which the separating agent for optical isomers is held in a column tube.
1. A separating agent for optical isomers which is used for separation of optical isomers in a sample comprising the optical isomers, which separating agent comprises a monolithic inorganic type carrier, and at least one of an amylose and an amylose derivative supported on the monolithic inorganic type carrier, wherein:
the monolithic inorganic type carrier comprises a porous body in which channels are formed through connection of cavities from one end to the other end of the monolithic inorganic type carrier;
the cavities each comprise a macropore and a meso pore formed on an inner wall surface of the macropore;
the macropore has a pore size of 0.5 to 30 μm and
the meso pore has a pore size of 23 to 25 nm.
2. The separating agent for optical isomers according to claim 1 , wherein the macropore has a pore size of 0.5 to 10 μm.
3. The separating agent for optical isomers according to claim 1 or 2 , wherein the macropore has a pore size of 1.0 to 6.0 μm.
4. The separating agent for optical isomers according to claim 1 , wherein the monolithic inorganic type carrier is mainly composed of silica.
5. The separating agent for optical isomers according to claim 1 , wherein the amylose derivative is one of an ester derivative of amylose and a carbamate derivative of amylose.
6. The separating agent for optical isomers according to claim 5 , comprising amylose tris(3,5-dimethylphenylcarbamate) supported on the monolithic inorganic type carrier.
7. A separation column for optical isomers comprising:
a column tube; and
the separating agent for optical isomers according to claim 1 which is held in the column tube.