Use of VEGF and homologues to treat neuron disorders
The present invention relates to neurological and physiological dysfunction associated with neuron disorders. In particular, the invention relates to the involvement of vascular endothelial growth factor (VEGF) and homologues in the aetiology of motor neuron disorders. The invention further concerns a novel, mutant transgenic mouse (VEGF m/m ) with a homozygous deletion in the hypoxia responsive element (HRE) of the VEGF promoter which alters the hypoxic upregulation of VEGF. These mice suffer severe adult onset muscle weakness due to progressive spinal motor neuron degeneration which is reminiscent of amyotrophic lateral sclerosis (ALS)—a fatal disorder with unknown aetiology. Furthermore, the neuropathy of these mice is not caused by vascular defects, but is due to defective VEGF-mediated survival signals to motor neurons. The present invention relates in particular to the isoform VEGF 165 which stimulates survival of motor neurons via binding to neuropilin-1, a receptor known to bind semaphorin-3A which is implicated in axon retraction and neuronal death, and the VEGF Receptor-2. The present invention thus relates to the usage of VEGF, in particular VEGF 165 , for the treatment of neuron disorders and relates, in addition, to the usage of polymorphisms in the VEGF promotor for diagnosing the latter disorders.
1. A method of enhancing survival of neurons in the central nervous system of a subject, the method consisting essentially of administering to the subject a VEGF-A protein in an amount and manner effective to enhance survival of neurons in the subject, wherein the subject has amyotrophic lateral sclerosis (ALS).
2. The method of claim 1 , wherein the VEGF-A protein is isoform VEGF 121 , isoform VEGF 145 , isoform VEGF 165 or isoform VEGF 189 .
3. The method of claim 1 , wherein the VEGF-A protein is administered intrathecally.
4. The method of claim 1 , wherein the VEGF-A protein is isoform VEGF 165 .