Resistance to downy mildew of onion caused by the fungus
The present invention relates to plants of the Allium cepa species, which are resistant to the downy mildew of onion caused by the fungus Peronospora destructor , due to a Pd resistance locus, wherein any fragment of a chromosome, comprising the Pd resistance locus, can be present homozygously in progeny without causing lethality. The present invention also encompasses plants of the Allium cepa species which are resistant to downy mildew of onion caused by the fungus Personospora destructor (Pd) due to Pd resistance locus present homozygously in the genome of the plants. The present invention also provides processes for obtaining plants resistant to downy mildew of onion, which are suitable for obtaining cultivated onions and shallots.
1. An onion or shallot plant of the Allium cepa species which is resistant to downy mildew of onion caused by the fungus Peronospora destructor (Pd), which plant comprises a Pd resistance locus present homozygously in the long arm of chromosome 3 in the genome of said plant, the Pd resistance locus having a sequence identical to a genomic segment of Allium roylei conferring resistance to downy mildew in A. roylei , wherein the Pd resistance locus comprises a 61 nucleotide amplification product with the selective amplification primers:
5′-GACTGCGTACATGCAGAAC (SEQ ID NO:5) and
5′ GATGAGTCCTGAGTAACTT (SEQ ID NO: 6) in the genome of said plant restricted with restriction enzymes PstI and MseI; and ligated with the following oligonucleotide adapters:
alpha 5′-CTCGTAGACTGCGTACATGCA (SEQ ID NO:1) CATCTGACGCATGT-5′ (SEQ ID NO:2), and
beta 5′-GACGATGAGTCCTGAG (SEQ ID NO:3) TACTCAGGACTCAT-5′ (SEQ ID NO:4).
2. An onion or shallot plant of the Allium cepa species which is resistant to downy mildew of onion caused by the fungus Peronospora destructor (Pd), which plant comprises a Pd resistance locus present homozygously in the long arm of chromosome 3 in the genome of said plant, the Pd resistance locus having a sequence identical to a genomic segment of Allium roylei conferring resistance to downy mildew in A. roylei , wherein the Pd resistance locus comprises a 151 nucleotide amplification product with the selective amplification primers:
5′-GACTGCGTACATGCAGAAG (SEQ ID NO:7) and
5′-GATGAGTCCTGAGTAAAAC (SEQ ID NO:8) in the genome of said plant restricted with restriction enzymes PstI and MseI; and ligated with the following oligonucleotide adapters:
alpha 5′-CTCGTAGACTGCGTACATGCA (SEQ ID NO:1) CATCTGACGCATGT-5′ (SEQ ID NO: 2), and
beta 5′-GACGATGAGTCCTGAG (SEQ ID NO:3) TACTCAGGACTCAT-5′ (SEQ ID NO:4).
3. An onion or shallot plant of the Allium cepa which is resistant to downy mildew of onion caused by the fungus Peronospora destructor (Pd), which plant comprises a Pd resistance locus present homozygously in the long arm of chromosome 3 in the genome of said plant, the Pd resistance locus having a sequence identical to a genomic segment of Allium roylei conferring resistance to downy mildew in A. roylei , wherein the Pd resistance locus comprises a 330 nucleotide amplification product with the selective amplification primers:
5′-GACTGCGTACATGCAGACA (SEQ ID NO:9) and
5′-GATGAGTCCTGAGTAACCA (SEQ ID NO:10) in the genome of said plant restricted with the restriction enzymes PstI and MseI; and ligated with the following oligonucleotide adapters:
alpha 5′-CTCGTAGACTGCGTACATGCA (SEQ ID NO: 1) CATCTGACGCATGT-5′ (SEQ ID NO:2), and
beta 5′-GACGATGAGTCCTGAG (SEQ ID NO:3) TACTCAGGACTCAT-5′ (SEQ ID NO:4).
4. An onion or shallot plant of the Allium cepa which is resistant to downy mildew of onion caused by the fungus Peronospora destructor (Pd), which plant comprises a Pd resistance locus present homozygously in the long arm of chromosome 3 in the genome of said plant, the Pd resistance locus having a sequence identical to a genomic segment of Allium roylei conferring resistance to downy mildew in A. roylei , wherein the Pd resistance locus comprises:
a) a 61 nucleotide amplification product with the primers:
5′-GACTGCGTACATGCAGAAC (SEQ ID NO:5) and
5′-GATGAGTCCTGAGTAACTT (SEQ ID NO:6), or
b) a 151 nucleotide amplification product with the primers:
5′-GACTGCGTACATGCAGAAG (SEQ ID NO:7) and
5′-GATGAGTCCTGAGTAAAAC (SEQ ID NO:8), or
c) a 330 nucleotide amplification product with the primers:
5′-GACTGCGTACATGCAGACA (SEQ ID NO:9) and
5′-GATGAGTCCTGAGTAACCA (SEQ ID NO:10), in the genome of said plant restricted by PstI and MseI and ligated to the following oligonucleotide adapters:
alpha 5′-CTCGTAGACTGCGTACATGCA (SEQ ID NO:1) CATCTGACGCATGT-5′ (SEQ ID NO:2), and
beta 5′-GACGATGAGTCCTGAG (SEQ ID NO:3) TACTCAGGACTCAT-5′ (SEQ ID NO:4).