Methods for determining the hair follicle inductive properties of a composition
The present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal and determining whether at least one hair follicle forms.
1. A method for determining hair inductive properties of a composition comprising:
injecting a composition comprising dissociated dermal cells and epidermal cells into the deep dermis of an immunocompromised mouse, wherein the dermal cells are from a first species and epidermal cells are from a second species and wherein the first species is different from the second species; and
determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla; and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.
2. The method of claim 1 , wherein the first species is mouse and the second species is human.
3. The method of claim 1 , wherein the first species is human and the second species is mouse.
4. The method of claim 1 , wherein from about one million to about five million cells are injected.
5. The method of claim 1 , wherein the composition further comprises a carrier.
6. The method of claim 1 , wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.
7. A method for determining hair inductive properties of a composition comprising:
injecting a composition comprising dissociated dermal cells and epidermal cells into the deep dermis of an immunocompromised mouse, wherein the dermal cells and epidermal cells are human; and
determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla; and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.
8. The method of claim 7 , wherein from about one to about five million cells are injected.
9. The method of claim 7 , wherein the composition further comprises a carrier.
10. The method of claim 7 , wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.