Granulysin peptides and methods of use thereof
View Patent ↗Granulysin peptides are small antimicrobial agents with potent activity. A pharmaceutical composition comprising granulysin peptides as an active agent is administered therapeutically to a patient for exfoliation, e.g. for the treatment of skin lesions.
1. A method for exfoliation of the skin of an individual, the method comprising:
applying to said skin a composition comprising a biologically active acylated granulysin peptide in a dose effective for topical cytolysis of keratinocytes and killing of P. acnes ; and an acceptable vehicle, wherein the granulysin peptide has a sequence selected from:
SEQ ID NO:2 AATRVSRTGRSRWRDWSRNFMR;
SEQ ID NO:3 ERVSRTGRSRWRDWSRNFMRRR;
SEQ ID NO:4 RRVSRTGRSRWRDWSRNFMRRR;
SEQ ID NO:5 RSRWRDVARNFMR;
SEQ ID NO:6 RSRWRDVARNFMR; SEQ ID NO:7 RSRWRDWSRNFMR;
SEQ ID NO:8 RSVSNAATRVSRTGRSRWRDWSRNFMR;
SEQ ID NO:9 TRVARTGRSRWRDWARNFMR;
SEQ ID NO:10 TRVDRTGRSRWRDWZRNFMR;
SEQ ID NO:11 TRVSRTGRSRWRDVSRNFMR;
SEQ ID NO:12 TRVSRTGRSRWRDWSRNFMR;
SEQ ID NO:14 TRVSRTGRSRWRDWSRNFMRAA;
SEQ ID NO:15 TRVSRTGRSRWRDWSRNFMRAAKKKKKKKK;
SEQ ID NO:16 TRVSRTGRSRWRDWSRNFMRN;
SEQ ID NO:17 TRVSRTGRSRWRDWSRNFMRNW;
topical cytolysis SEQ ID NO:18 TRVSRTGRSRWRDWSRNFMRNY;
SEQ ID NO:19 TRVSRTGRSRWRDWSRNFMRRR;
SEQ ID NO:20 TRVSRTGRSRWRDWSRNWMRRR;
SEQ ID NO:21 TRVZRTGRSRWRDWDRNFMR.
2. The method of claim 1 , wherein the peptide is (SEQ ID NO:12) TRVSRTGRSRWRDWSRNFMR or (SEQ ID NO:5) RSRWRDVARNFMR.
3. The method of claim 2 , wherein the peptide is a D amino acid form.
4. The method of claim 1 , wherein the acylated peptide comprises a lauryl group.
5. The method of claim 1 , wherein said skin comprises acne lesions.