Methods and means for producing hyaluronan
View Patent ↗The present invention relates to fungal cells and fungi which synthesize hyaluronan and to methods for preparing such fungi, and also to methods for preparing hyaluronan with the aid of these fungal cells or fungi. Furthermore, the present invention relates to the use of fungi for preparing hyaluronan and to food or feed which comprises hyaluronan.
1. A fungal cell of the systematic order Agaricales systematic class Basidiomycetes comprising a foreign nucleic acid molecule coding for a hyaluronan synthase, wherein said fungal cell synthesizes hyaluronan.
2. A fungus comprising the fungal cell of claim 1 .
3. A propagation material of a fungus of claim 2 , wherein said propagation material comprises said nucleic acid molecule coding for a hyaluronan synthase.
4. A method for preparing a fungus which synthesizes hyaluronan, comprising
a) transforming a fungal cell of the systematic order Agaricales systematic class Basidiomycetes with a nucleic acid molecule coding for a hyaluronan synthase, wherein said nucleic acid is integrated into the genome of said fungal cell; and
b) regenerating a fungus from the fungal cell of step a).
5. A composition comprising components of the fungal cell of claim 1 .
6. The method of claim 4 , wherein fungi are generated from the fungi of step b).
7. A composition comprising components of the fungal cell of claim 1 .
8. A composition comprising components of the fungus of claim 2 .
9. A composition comprising components of the fungi obtainable by the method of claim 4 .
10. The fungal cell of claim 1 , wherein said fungal cell is of the systematic family Agaricaceae.
11. The method of claim 4 , wherein said fungal cell is of the systematic family Agaricaceae.
12. The fungal cell of claim 1 , wherein said fungal cell is of the genus Agaricus.
13. The method of claim 4 , wherein said fungal cell is of the genus Agaricus.
14. The fungal cell of claim 1 , wherein said fungal cell is of the species Agaricus bisporus.
15. The method of claim 4 , wherein said fungal cell is of the species Agaricus bisporus.
16. The fungal cell of claim 12 , wherein the foreign nucleic acid comprises a nucleic acid molecule that encodes the amino acid sequence of SEQ ID NO: 2 or 4.
17. The method of claim 13 , wherein the nucleic acid molecule encodes the amino acid sequence of SEQ ID NO: 2 or 4.
18. The fungal cell of claim 14 , wherein the foreign nucleic acid comprises a nucleic acid molecule that encodes the amino acid sequence of SEQ ID NO: 2 or 4.
19. The method of claim 15 , wherein the nucleic acid molecule encodes the amino acid sequence of SEQ ID NO: 2 or 4.