Capillary electrophoresis apparatus
View Patent ↗A capillary electrophoresis apparatus capable of efficiently using a polymer in a polymer container is provided. The present invention relates to a mechanism for efficiently using the polymer in the polymer container in a capillary electrophoresis apparatus for the electrophoresis with a separation medium filled in a capillary. According to the present invention, since the polymer in the polymer container is not contacted with the air, deterioration of the polymer by oxidation can be prevented.
1. A capillary electrophoresis apparatus comprising a capillary, a pump unit for filling the capillary with a separation medium, an irradiation and detection unit having a light source for irradiating an exciting light to a capillary detection area and a signal detecting mechanism for detecting a fluorescence from the capillary detection area, and a power supply unit for applying a voltage between a capillary end of sample injection side electrode and an anode,
wherein the pump unit has a polymer container for storing a polymer as the separation medium, the polymer container being a sealed container having an opening and a cap to be engaged with the opening, the polymer container being formed of a flexible material,
the pump unit having a syringe and a polymer block which has a pipe for connection between the syringe, the polymer container and the capillary, and
wherein:
the polymer is sealed in the polymer container,
the pump unit is configured to fill the capillary with the polymer separation medium via suction of the polymer from the polymer container, and to cause atmospheric pressure to crush the container by reducing pressure in the container so that air does not flow into the polymer container,
the opening is sealed with a fastening cap with a tube provided therethrough when an open end of the tube is inserted through the opening of the polymer container, and
the open end of the tube inserted through the opening of the polymer container is configured to reach only partially into the polymer container.
2. The capillary electrophoresis apparatus according to claim 1 , wherein the polymer container is provided with a vent and a cap to be engaged with the vent.
3. The capillary electrophoresis apparatus according to claim 1 , wherein the polymer container is formed as a pouch or a bag made of a plastic material.