Preparation and use of biofilm-degrading, multiple-specificity, hydrolytic enzyme mixtures
The present invention relates to isolated structures containing degradative enzymes produced from a marine organism. The enzymes produced are based on the carbon source upon which the marine organism is growing. The enzymes are found in structures that can be isolated such that the degradative enzymes are easily harvested.
1. A composition comprising one or more isolated degradosomes from Microbulbifer 2-40 comprising at least one degradative enzyme, wherein the enzyme cleaves linkages between monomers in a polysaccharide.
2. The composition of claim 1 , wherein the enzyme acts endolytically.
3. The composition of claim 1 , wherein the enzyme acts exolytically.
4. The composition of claim 1 , wherein the polysaccharide is carboxymethylcellulose.
5. The composition of claim 1 , wherein the polysaccharide is alginate.
6. The composition of claim 1 , wherein the polysaccharide is araban.
7. The composition of claim 1 , wherein the polysaccharide is carrageenan.
8. The composition of claim 1 , wherein the polysaccharide is cellulose.
9. The composition of claim 1 , wherein the polysaccharide is chitin.
10. The composition of claim 1 , wherein the polysaccharide is β-glucan.
11. The composition of claim 1 , wherein the polysaccharide is pectin.
12. The composition of claim 1 , wherein the polysaccharide is laminarin.
13. The composition of claim 1 , wherein the polysaccharide is pullulan.
14. The composition of claim 1 , wherein the polysaccharide is starch.
15. The composition of claim 1 , wherein the polysaccharide is xylan.
16. The composition of claim 1 , wherein the polysaccharide is agar.
17. The composition of claim 1 , wherein the composition comprises at least two degradative enzymes.