IP Library Granted Patent US 8,114,642
Granted Patent B2
US 8,114,642 · App. 11/823,769 · Granted Feb 14, 2012

Method for producing vitamin K2 from culture of

Assignees: Japan Bio Science Laboratory Co., Ltd.; Daiwa Kasei K. K.
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Quick Facts
Patent No.
US 8,114,642
App. No.
11/823,769
Granted
Feb 14, 2012
Kind
B2
Abstract

A method for producing vitamin K2 comprising: culturing Bacillus natto in a liquid medium to obtain a Bacillus natto culture or culture supernatant thereof, wherein the culture or culture supernatant contains vitamin K2; treating the culture or culture supernatant with at least one coagulant selected from the group consisting of an inorganic coagulant and a cationic polymer coagulant, with the proviso that the coagulant excludes chitosan, so as to obtain a treated coagulant to which vitamin K2 is absorbed or aggregated; separating the treated coagulant from the treated culture or culture supernatant, and obtaining an oily product that contains vitamin K2 from the separated coagulant.

Claims (9)

1. A method for producing vitamin K2, comprising:

culturing Bacillus natto in a liquid medium to obtain a Bacillus natto culture or culture supernatant thereof, wherein the culture or culture supernatant contains vitamin K2;

treating the Bacillus natto culture or culture supernatant with an inorganic coagulant in combination with a phosphorus-containing coagulant, wherein the inorganic coagulant is calcium chloride and the phosphorus-containing coagulant is at least one selected from the group consisting of disodium hydrogenphosphate, sodium dihydrogenphosphate, potassium dihydrogenphosphate and tripotassium phosphate, so as to form a coagulant to which vitamin K2 from the Bacillus natto culture or culture supernatant is absorbed or aggregated;

separating the coagulant to which vitamin K2 is absorbed or aggregated from the treated culture or culture supernatant;

treating the coagulant to which vitamin K2 is absorbed or aggregated with an organic solvent for extraction;

collecting an organic solvent extract;

removing the organic solvent from the organic solvent extract; and

obtaining an oily product that contains vitamin K2 from the extract from which the organic solvent has been removed.

2. The method of claim 1 , wherein the separation is performed by filtration so as to obtain the coagulant to which vitamin K2 is absorbed or aggregated as a filtration residue.

Assignments (4)
MERGER Recorded Dec 3, 2013
From: DAIWA KASEI K.K.
To: AMANO ENZYME INC.
Reel/Frame 031705/0803 →
MERGER Recorded Oct 21, 2013
From: KASEI K.K.
To: AMANO ENZYME INC.
Reel/Frame 031441/0215 →
CHANGE OF ASSIGNEE ADDRESS Recorded Mar 11, 2013
From: JAPAN BIO SCIENCE LABORATORY CO., LTD.
To: JAPAN BIO SCIENCE LABORATORY CO., LTD.
Reel/Frame 029958/0854 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 1, 2011
From: TAKAOKA, SHINSAKU; YOSHIDA, MASAHARU
To: JAPAN BIO SCIENCE LABORATORY CO., LTD.; DAIWA KASEI K. K.
Reel/Frame 027154/0593 →
Priority Claims (1)
JP 2004-378455 · Dec 28, 2004 · national
Continuity (2)
Continuation In Part 11296942 · Dec 8, 2005
Related Publication 20070254347A1 · Nov 1, 2007