Composite device for the repair or regeneration of tissue
The present invention is directed to composite devices comprising a tissue-engineering scaffold and an anionic polysaccharide/structural protein coating. The composite devices are useful for the reparation and regeneration of tissue, particularly in the treatment of chronic wounds.
1 . A composite device for the treatment of chronic wounds comprising, a tissue-engineering scaffold and an anionic polysaccharide/structural protein coating, wherein said tissue-engineering scaffold is a biocompatible, biodegradable polymer and said anionic polysaccharide is a mucopolysaccharide or polycarboxylate.
2 . The composite device of claim 1 , wherein said scaffold is selected from the group consisting of sponges, foams, perforated films and textiles.
3 . The composite device of claim 2 , wherein said scaffold is a textile selected from the group consisting of weaves, knits, braids, meshes and nonwovens.
4 . The composite device of claim 3 , wherein said scaffold is a nonwoven comprising a copolymer of glycolide and lactide.
5 . The composite device of claim 3 , wherein said scaffold is a nonwoven comprising a copolymer of glycolide and lactide having a mole ratio of glycolide to lactide of about 90:10.
6 . The composite device of claim 2 , wherein said scaffold is a foam comprising an elastomeric copolymer.
7 . The composite device of claim 6 , wherein said elastomeric copolymer comprises epsilon-caprolactone and glycolide.
8 . The composite device of claim 7 , wherein said elastomeric copolymer comprises a mole ratio of epsilon-caprolactone to glycolide of from about 30:70 to about 70:30.
9 . The composite device of claim 1 , wherein said anionic polysaccharide is selected from the group consisting of heparin, hyaluronic acid, heparan sulphate, chondroitin sulphate, alginates, oxidized regenerated cellulose and oxidized cellulose.
10 . The composite device of claim 1 , wherein said anionic polysaccharide is selected from the group consisting of oxidized regenerated cellulose, oxidized cellulose, hyaluronic acid and chondroitin sulphate.
11 . The composite device of claim 1 , wherein said anionic polysaccharide is oxidized regenerated cellulose.
12 . The composite device of claim 1 , wherein said structural protein is selected from the group consisting of collagen, fibrin, fibronectin and laminin.
13 . The composite device of claim 1 , wherein said structural protein is collagen.
14 . The composite device of claim 1 , wherein said anionic polysaccharide/structural protein has a mole ratio of anionic polysaccharide to structural protein from about 10:90 to about 90:10.
15 . The composite device of claim 1 , wherein said scaffold is a foam comprising an elastomeric copolymer of epsilon-caprolactone and glycolide having a mole ratio of epsilon-caprolactone to glycolide from about 35:65 to about 65:35, and said anionic polysaccharide/structural protein coating is oxidized regenerated cellulose/collagen having a mole ratio from about 10:90 to about 90:10.
16 . The composite device of claim 1 , wherein said scaffold is a foam comprising an elastomeric copolymer of epsilon-caprolactone and glycolide having a mole ratio of epsilon-caprolactone to glycolide of about 35:65, and said anionic polysaccharide/structural protein coating is oxidized regenerated cellulose/collagen having a mole ratio of about 45:55.
17 . The composite device of claim 1 , wherein said scaffold is a nonwoven comprising a copolymer of glycolide and lactide having a mole ratio of about 90:10, and said anionic polysaccharide/structural protein coating is oxidized regenerated cellulose/collagen having a mole ratio of about 45:55.
18 . A method of treating a wound comprising,
a) contacting a wound with one or more composite devices, wherein said device(s) comprise a biocompatible, biodegradable tissue-engineering scaffold having an anionic polysaccharide/structural protein coating,
b) allowing said devices and said wound to remain in contact with each other, and
c) covering or closing said wound,
wherein said wound is treated.
19 . A kit comprising, a sterile composite device of claim 1 .
20 . The composite device of claim 1 , wherein said device is capable of increasing the presence of activated macrophages in said wound.