Two-photon dyes for real-time imaging of lipid rafts
View Patent ↗Two-photon dyes for real-time imaging of lipid rafts are provided. The two-photon dyes emit fluorescence with high intensity in the environments of cell membranes and can be used to clearly discriminate the hydrophobic and hydrophilic domains of the cell membranes. Therefore, the use of the two-photon dyes enables satisfactory imaging of actual lipid raft domains. In addition, since the two-photon dyes show no cytotoxicity, they can be suitably used in bioimaging applications, for example, real-time imaging of lipid rafts over a long period of time.
1. A two-photon dye for real-time imaging of lipid rafts, represented by Formula 1:
wherein R 1 is a C 5 -C 17 alkyl group,
n is 0 or 1, and
R 2 is —COOH, —CH 2 SO 3 Na or
(R 3 is a C 5 -C 17 alkyl group).
2. The two-photon dye according to claim 1 , wherein the compound of Formula 1 is selected from the group consisting of the following compounds (2):
3. The two-photon dye according to claim 1 , wherein the compound of Formula 1 is the compound represented by Formula 3:
4. The two-photon dye according to claim 1 , wherein the compound of Formula 1 is selected from the group consisting of the following compounds (4):
5. The two-photon dye according to claim 1 , wherein the compound of Formula 1 is selected from the group consisting of the following compounds (5):
6. The two-photon dye according to claim 1 , wherein the two-photon dye has a two-photon absorption efficiency not lower than 20 GM in living cells, is two-photon excited by a light source at 750-1,000 nm, and emits fluorescence ranging from 400 to 700 nm.
7. The two-photon dye according to claim 1 , wherein the two-photon dye has a two-photon absorption efficiency not lower than 150 GM, and emits fluorescence of 430-470 nm in a hydrophobic environment and fluorescence of 470-530 nm in a hydrophilic environment.