Cytochrome c synthesis inhibitors
The invention provides methods for identifying a compound that inhibits cytochrome c synthesis. This invention further provides a method for the high throughput screening of compounds that inhibit cytochrome c synthesis.
1. A method for identifying a compound that inhibits cytochrome c synthesis in a bacterial cell, the method comprising: a. contacting a transfected bacterial cell with a compound, the bacterial cell being transfected with a first expression vector encoding Ccm proteins and a second expression vector encoding a cytochrome c reporter protein, wherein the bacterial cell has a chromosomal disruption in the region encoding the Ccm proteins; and b. determining the amount of cytochrome c reporter protein produced in the presence of the compound relative to the amount produced in the absence of the compound, wherein a decrease in amount is an indication that the compound inhibits the synthesis of cytochrome c.
2. The method of claim 1 , wherein the transfected bacterial is a gamma proteobacterium cell.
3. The method of claim 2 , wherein the transfected bacterial cell is an Escherichia coli cell.
4. The method of claim 1 , wherein the Ccm proteins coded by the first expression vector are from a gamma proteobacterium.
5. The method of claim 4 , wherein the Ccm protiens are the CcmA-H proteins of Escherichia coli.
6. The method of claim 1 , wherein the second expression vector encodes a cytochrome c:alkaline phosphate fusion protien or a cytochrome c:6xHis fusion protien.
7. The method of claim 6 , wherein the cytochrome c protien is cytochrome c 4 from Brodetella pertussis.
8. The method of claim 1 , wherein the coding region of the first expression vector is operably linked to a first inducible promoter and the coding region of the second expression vector is operably linked to a second inducible promoter.
9. The method of claim 8 , wherein the first promoter is induced by IPTG and the second promoter is induced by arabinose.
10. The method of claim 1 , wherein the transfected bacterial cell grows in the presence of exogenous amino levulinic acid (ALA).
11. The method of claim 1 , wherein the transfected bacterial cell further comprises a chromosomal disruption in the region encoding the HemA protein.