Sulfotransferase 2B1 pharmacogenetics
View Patent ↗Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as are sulfotransferase allozymes. Methods for determining the sulfonator status of a subject also are described. In addition, methods for predicting the therapeutic efficacy of a compound in a subject are described, as are methods for estimating the dose of a compound to be administered to a subject.
1. An isolated nucleic acid molecule consisting of: (a) twenty to 100 contiguous nucleotides of SEQ ID NO: 15, wherein said nucleic acid molecule includes at least one nucleotide selected from positions 482, 901, and 1364 of SEQ ID NO: 15, with the proviso that the nucleotide at position 482 is cytosine, the nucleotide at position 901 is adenine, and/or the nucleotide at position 1364 is thymine; or (b) the complement of (a).
2. The isolated nucleic acid molecule of claim 1 , wherein said isolated nucleic acid molecule is 20 to 50 nucleotides in length.
3. A vector comprising the nucleic acid molecule of claim 1 .
4. The vector of claim 3 , wherein said nucleic acid molecule is 20 to 50 nucleotides in length.
5. An article of manufacture comprising a substrate, wherein said substrate comprises the isolated nucleic acid molecule of claim 1 .
6. An isolated nucleic acid molecule consisting of: a) twenty to 100 contiguous nucleotides of SEQ ID NO:15, wherein said nucleic acid molecule includes one or more of nucleotides 482, 901, and 1364 of SEQ ID NO:15, with the proviso that the nucleotide at position 482 is cytosine, the nucleotide at position 901 is adenine, and/or the nucleotide at position 1364 is thymine; or b) the complement of a), and, with respect to a) or b), a label.
7. The isolated nucleic acid molecule of claim 6 , wherein said label is a fluorescent moiety.
8. The isolated nucleic acid molecule of claim 6 , wherein said label is biotin.
9. The isolated nucleic acid of molecule of claim 6 , wherein said isolated nucleic acid molecule is 20 to 50 nucleotides in length.
10. A vector comprising the nucleic acid molecule of claim 6 .
11. The vector of claim 10 , wherein said nucleic acid molecule is 20 to 50 nucleotides in length.
12. An article of manufacture comprising a substrate, wherein said substrate comprises the isolated nucleic acid molecule of claim 6 .