Virus vaccination and treatment methods with OX40 agonist compositions
The invention relates to compositions and methods that employ OX40 (CD134), a TNFR superfamily protein, agonists. The invention includes among other things administering an OX40 agonist alone or in combination with a viral antigen, or live or attenuated virus, to treat a viral infection, or for vaccination or immunization.
1. A method of inducing a CD8+ T cell response to protect a subject against poxvirus replication, comprising administering to the subject a CD8+ T cell specific poxvirus antigen and an amount of an OX40 (CD 134) agonist sufficient to induce the CD8+ T cell response of the subject, thereby protecting the subject against the poxvirus replication wherein the CD8+ T cell specific poxvirus antigen is a cell free CD8+ peptide.
2. A method of treating a subject having a viral infection caused by a poxvirus, comprising administering to the subject a CD8+ T cell specific poxvirus antigen and an amount of an OX40 agonist sufficient to enhance or augment an anti-viral CD8+ T cell response of the subject, thereby protecting the subject against the viral infection, wherein the CD8+ T cell specific poxvirus antigen is a cell free CD8+ peptide.
3. The method of claim 1 , wherein the OX40 (CD134) agonist comprises an antibody.
4. The method of claim 3 , wherein the antibody is mammalian, primatized, humanized or fully human.
5. The method of claim 3 , wherein the antibody is monoclonal or polyclonal.
6. The method of claim 2 , wherein the viral infection is acute.
7. The method of claim 1 , wherein the subject is a mammal.
8. The method of claim 1 , wherein the subject is a human.
9. The method of claim 1 or 2 , wherein the CD8+ T cell specific poxvirus antigen comprises a vaccinia virus, Molluscum contagiosum , variola major or variola minor smallpox virus, cow pox, camel pox, sheep pox, or monkey pox antigen.
10. The method of claim 9 , wherein the CD8+ T cell specific poxvirus antigen is a vaccinia virus antigen selected from a B8R, A3L, A8R, A23R or B2R antigen.
11. The method of claim 2 , wherein the poxvirus comprises a vaccinia virus, Molluscum contagiosum , variola major or variola minor smallpox virus, cow pox, camel pox, sheep pox, or monkey pox.
12. The method of claim 1 , wherein the method reduces the amount of a poxvirus protein.
13. The method of claim 1 or 2 , wherein the OX40 (CD134) agonist is administered prior to, substantially contemporaneously with or following administration of the CD8+ T cell specific poxvirus antigen.
14. The method of claim 1 or 2 , wherein the OX40 (CD 134) agonist is administered prior to, substantially contemporaneously with or following exposure to or infection of the subject with the poxvirus.
15. A method of inducing an antiviral CD8+ T cell response in a subject with a viral infection caused by a poxvirus to protect the subject against poxvirus replication, comprising administering to the subject a CD8+ T cell specific poxvirus antigen and an amount of OX40 (CD134) agonist sufficient to induce the antiviral CD8+ T cell response in the subject to protect the subject against poxvirus replication, wherein the CD8+ T cell specific poxvirus antigen is a cell free CD8+ peptide and wherein the CD8+ T cell specific poxvirus antigen is administered prior to administration of the OX40(CD134) agonist to the subject.
16. The method of claim 1 , wherein the CD8+ T cell specific poxvirus antigen consists of a class I restricted CD8+ T cell peptide epitope.