Methods and compositions for determining predisposition to inflammation-mediated cardiovascular disease
View Patent ↗The present invention provides methods and compositions for detecting a predisposition to an inflammation-mediated cardiovascular disease in a human subject by detecting a level of leukotriene C4 synthase (LTC4S) gene product in a sample from a human subject indicative of a predisposition to an inflammation-mediated cardiovascular disease or detecting the presence or absence of an allele of LTC4S indicative of a predisposition to an inflammation-mediated cardiovascular disease. In addition, the present invention also provides kits for practicing the methods.
1. A method of determining a predisposition to atherosclerosis in a female human subject, said method comprising:
assaying the promoter region of leukotriene C4 synthase (LTC4S) in a biological sample of the subject;
detecting the presence of a cytosine at position −444 in said promoter region; and
determining a predisposition to atherosclerosis based on said presence of said cytosine.
2. The method of claim 1 , wherein the assaying comprises a polymerase chain reaction.
3. The method of claim 1 , wherein the assaying comprises hybridization.
4. The method of claim 2 , wherein the assaying further comprises sequencing of the product of the polymerase chain reaction.
5. A method of determining predisposition for the presence of Coronary Artery Calcium (CAC) in a female human subject comprising:
assaying the promoter region of leukotriene C4 synthase (LTC4S) in a biological sample of the subject;
detecting the presence of a cytosine at position −444 in said promoter region; and
determining the predisposition for the presence of CAC in the subject based on said presence of said cytosine.
6. The method of claim 5 , wherein the assaying comprises a polymerase chain reaction.
7. The method of claim 6 , wherein the assaying further comprises sequencing of the product of the polymerase chain reaction.
8. The method of claim 5 , wherein the assaying comprises hybridization.
9. A method of determining a predisposition to atherosclerosis in a female human subject comprising:
assaying the promoter region of leukotriene C4 synthase (LTC4S) in a biological sample from the subject;
detecting the presence of a cytosine at position −444 in said promoter region; and
determining predisposition to a high mean carotid artery intimal-medial thickness in the subject based on said presence of said cytosine wherein high mean carotid artery intimal-medial thickness indicates a predisposition to atherosclerosis.
10. The method of claim 9 , wherein the assaying comprises a polymerase chain reaction.
11. The method of claim 10 , wherein the assaying further comprises sequencing of the product of the polymerase chain reaction.
12. The method of claim 9 , wherein the assaying comprises hybridization.