Reagents and methods for detecting
This invention provides compositions and methods for detecting Neisseria gonorrhoeae in a sample. This invention also provides related reaction mixtures, kits, systems, and computers.
1. A kit, comprising:
(a) at least one oligonucleotide consisting of at least 90% sequence identity with SEQ ID NO: 38 or with full complements thereof;
(b) at least one oligonucleotide selected from the group consisting of: SEQ ID NOS: 39-45, a substantially identical variant thereof wherein the variant has at least 90% sequence identity to one of SEQ ID NOS: 39-45, and with full complements of SEQ ID NOS: 39-45 and the variant; and
(c) instructions for determining a presence of Neisseria gonorrhoeae in a sample by monitoring binding between nucleic acids and/or amplicons thereof from the sample and the oligonucleotide, wherein the presence of Neisseria gonorrhoeae in the sample is unknown or unsubstantiated.
2. The kit of claim 1 , further comprising one or more nucleic acid detection reagents that detectably bind to a Chlamydia trachomatis nucleic acid.
3. The kit of claim 2 , further comprising at least one enzyme.