IP Library Granted Patent US 7,892,788
Granted Patent B2
US 7,892,788 · App. 11/883,778 · Granted Feb 22, 2011

Hydrophobin fusion products, production and use thereof

Assignee: BASF SE
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Quick Facts
Patent No.
US 7,892,788
App. No.
11/883,778
Granted
Feb 22, 2011
Kind
B2
Abstract

Polypeptides of the general structural formula (I) X n —C 1 —X 1-50 —C 2 —X 0-5 —C 3 —X 1-100 —C 4 —X 1-100 —C 5 —X 1-50 —C 6 —C 0-5 —C 7 —X 1-50 —C 8 —X m   (I) production and use thereof.

Claims (16)

1. A polypeptide of the general structural formula (I)

X n —C 1 —X 1-50 —C 2 —X 0-5 —C 3 —X 1-100 —C 4 —X 1-100 —C 5 —X 1-50 —C 6 —X 0-5 —C 7 —X 1-50 C 8 —X m   (I)

wherein X is any of the 20 naturally occurring amino acids,

n and m are numbers between 0 and 500,

C is cysteine,

with the proviso that at least one of the peptide sequences abbreviated as X n or X m is a peptide sequence of at least 20 amino acids in length that is not linked naturally to a hydrophobin and X n or X m comprises an amino acid sequence selected from SEQ ID NOs: 16 and 18, and wherein the polypeptide changes the contact angle of a drop of water on a glass surface by at least 20° after coating the glass surface with the polypeptide.

2. The polypeptide of claim 1 , wherein the structural formula (I) comprises a Class I hydrophobin.

3. The polypeptide of claim 1 , wherein the structural formula (I) comprises a hydrophobin selected from the group consisting of dewA, rodA, sc3, hypA, hypB, basf1, and basf2.

4. The polypeptide of claim 1 , wherein the structural formula (I) comprises a sequence selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, and 14.

5. The polypeptide of claim 1 , wherein X n or X m is a (His) 4-10 sequence.

6. The polypeptide of claim 1 , wherein the structural formula (I) comprises polypeptides selected from the group consisting of SEQ ID NO: 20, 22, and 24.

7. A method for coating a surface with a hydrophobin, the method comprising coating the surface with the polypeptide of claim 1 .

8. A nucleic acid coding for the polypeptide of claim 1 .

9. A method of producing the polypeptide of claim 1 , the method comprising expressing in a host organism a nucleic acid encoding the polypeptide of claim 1 and isolating the protein obtained.

10. The method of claim 9 , wherein the host organism is E. coli.

11. The method of claim 9 , wherein the protein is isolated after purification.

Assignments (2)
CHANGE OF NAME Recorded Oct 19, 2010
From: BASF AKTIENGESELLSCHAFT
To: BASF SE
Reel/Frame 025166/0622 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 17, 2007
From: SUBKOWSKI, THOMAS; KAROS, MARVIN; LEMAIRE, HANS-GEORG; BARG, HEIKO; BOLLSCHWEILER, CLAUS
To: BASF AKTIENGESELLSCHAFT
Reel/Frame 019709/0891 →
Priority Claims (2)
DE 10 2005 005 737 · Feb 7, 2005 · national
DE 10 2005 007 480 · Feb 17, 2005 · national
Continuity (1)
Related Publication 20090104663A1 · Apr 23, 2009