Aprotinin polypeptides for transporting a compound across the blood-brain barrier
The invention relates to improvements in the field of drug delivery. More particularly, the invention relates to polypeptides derived from aprotinin and from aprotinin analogs as well as conjugates and pharmaceutical compositions comprising these polypeptides or conjugates. The present invention also relates to the use of these polypeptide for transporting a compound or drug across the blood-brain barrier of a mammal and in the treatment and diagnosis of neurological diseases.
1. A polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of Angiopep-1 (SEQ ID NO.:67), wherein said polypeptide has a substitution at the position corresponding to the cysteine at position 7 of the Angiopep-1 sequence, or a pharmaceutically acceptable salt thereof.
2. The polypeptide of claim 1 , wherein said substitution is a serine substitution.
3. The polypeptide of claim 1 , wherein said polypeptide is capable of crossing the blood-brain barrier or an in vitro model thereof.
4. The polypeptide of claim 1 , where said percent identity is at least 85%.
5. The polypeptide of claim 4 , wherein said percent identity is at least 90%.
6. The polypeptide of claim 2 comprising the sequence of Angiopep-2 (SEQ ID NO.:97).
7. The polypeptide of claim 1 , wherein said polypeptide is a fusion protein.
8. The fusion protein of claim 7 comprising leptin.
9. A conjugate comprising:
(a) the polypeptide of claim 1 ; and
(b) a peptide or protein, wherein said peptide or said protein is conjugated to said polypeptide.
10. The conjugate of claim 9 , wherein said protein comprises leptin.
11. A composition comprising the polypeptide of claim 1 .
12. A pharmaceutical composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable carrier.
13. A method of treating a patient suffering from obesity, said method comprising administering to said patient a polypeptide of claim 8 in an effective amount.
14. A method of treating a patient suffering from obesity, said method comprising administering to said patient a conjugate of claim 10 in an effective amount.