Nanothin polymer films with selective pores and method of use thereof
View Patent ↗A method of preparing nanothin polymer films having uniform and selectively sized pores utilizing pore forming templates. Lipids and pore forming templates are dissolved into a first solution. The solvent is removed thereby creating a lipid bilayer with pore forming templates dispersed throughout. The bilayer is hydrated and monomers and crosslinkers are added to create a second solution. A nanothin film with pore forming templates is created through polymerization of said second solution. The pore forming templates are dissolved into a third solution by addition of a chemical in which the pore forming template is soluble, but the lipid bilayer is insoluble. This third solution is separated from the mixture leaving a nanothin polymer film with pores of a uniform thickness and surface area. In summary, the guided assembly method presented here suggests a new general strategy for the fabrication of nanothin materials with controlled permeability, which provides improvements over the currently existing technology.
1. A method of producing a polymer film with pores, comprising:
providing a lipid;
providing a pore forming template;
dissolving the lipid and the pore forming template into a first solution;
drying the first solution in order to create a lipid film with the pore forming templates dispersed throughout;
hydrating the lipid film in order to create a second solution;
extruding the second solution to form a unilamellar, uniform lipid film;
providing a monomer in an effective amount so that a pore forming template spans a thickness of a formed polymer film in a lipid bilayer of the lipid film;
providing a crosslinker;
adding the monomer and the crosslinker to the extruded second solution;
polymerizing the second solution in order to form the lipid bilayer with an interior, hydrophobic, polymer film with the pore forming templates dispersed throughout, wherein the polymer film has a thickness so that the pore forming template spans the thickness of the polymer film;
dissolving the pore forming templates into a third solution by addition of a solvent; and
removing the third solution by separation in order to leave a polymer film with pores of a uniform thickness and surface area.
2. The method of claim 1 , wherein the pore forming template is an inert molecule that is chemically degradable and hydrophobic.
3. The method of claim 2 , wherein the pore forming template is glucose pentaacetate.
4. The method of claim 2 , wherein the pore forming template is glucose pentabenzoate.
5. The method of claim 2 , wherein the extruding step is repeated at least 10 times.
6. The method of claim 5 , wherein the monomer is styrene.
7. The method of claim 5 , wherein the cross linker is divinylbenzene.
8. The method of claim 7 , wherein the solvent is a detergent solution.
9. The method of claim 5 , wherein dissolving the pore forming templates into a third solution by addition of a solvent further comprises:
adding a detergent solution;
adding an aqueous sodium hydroxide solution;
incubating for 0.5 hours; and
adjusting pH to 7 with hydrochloric acid.
10. A method of producing a polymer film with pores, comprising:
providing a lipid;
providing a pore forming template;
dissolving the lipid and the pore forming template into a first solution;
drying the first solution in order to create a lipid film with the pore forming templates dispersed throughout;
providing a monomer in an effective amount so that a pore forming template spans a thickness of a formed polymer film in a lipid bilayer of the lipid film;
providing a crosslinker;
adding the monomer and the crosslinker to the lipid film;
hydrating the lipid film in order to create a second solution;
extruding the second solution to form a unilamellar, uniform lipid film;
polymerizing the second solution in order to form the lipid bilayer with an interior, hydrophobic, polymer film with the pore forming templates dispersed throughout, wherein the polymer film has a thickness so that the pore forming template spans the thickness of the polymer film;
dissolving the pore forming templates into a third solution by addition of a solvent; and
removing the third solution by separation in order to leave a polymer film with pores of a uniform thickness and surface area.