Phosphorylated waxy potato starch
View Patent ↗The invention relates to potato starches having an amylose content of less than 10% by weight, a phosphate content in the C6 position of between 35 and 100 nmol of phosphate per milligram of starch and a content of side chains having a DP of from 12 to 19 which is elevated as compared with that in potato starch from corresponding wild-type potato plants.
1. A potato starch comprising an amylose content as measured by the method of Hovenkamp-Hermelink et al., of less than 10% by weight, a phosphate content in the C6 position of between 35 and 100 nmol of phosphate per milligram of starch (dry weight), and a proportion of side chains having a degree of polymerization (DP) of from 12 to 19 which is increased to 125%-200% as compared with potato starch from corresponding wild-type potato plants.
2. The potato of claim 1 , wherein said phosphate content in the C6 position is between 40 and 85 nmol of phosphate per milligram of starch (dry weight).
3. The potato starch of claim 1 , wherein said phosphate content in the C6 position is between 45 and 70 nmol of phosphate per milligram of starch (dry weight).
4. The potato starch of claim 1 , wherein said phosphate content in the C6 position is between 50 and 65 nmol of phosphate per milligram of starch (dry weight).
5. The potato starch of claim 1 , wherein said amylose content, as measured by the method of Hovenkamp-Hermelink et al. is less than 5% by weight.
6. The potato starch of claim 1 , wherein said amylose content, as measured by the method of Hovenkamp-Hermelink et al., is less than 3% by weight.
7. The potato starch of claim 1 , wherein the proportion of side chains having a DP of from 12 to 19 is increased to 130%-480% as compared with potato starch from corresponding wild-type potato plants.
8. The potato starch of claim 1 , wherein the proportion of side chains having a DP of from 63 to 123 is reduced as compared with potato starch from corresponding wild-type potato plants.
9. The potato starch of claim 8 , wherein the proportion of side chains having a DP of from 63 to 123 is reduced to 50%-95% as compared with potato starch from corresponding wild-type potato plants.
10. The potato starch of claim 1 , wherein said phosphate content in the C6 position is elevated by 415%-520% as compared with that in potato starch from corresponding wild-type plants.
11. A derivatized potato starch comprising the potato starch of claim 1 , wherein the derivatized potato starch is obtainable by subjecting the potato starch to a temperature treatment of 120° C.-140° C. in a dry system.
12. A derivatized potato starch comprising the potato starch of claim 1 , wherein the derivatized potato starch is obtainable by treating the potato starch with acid in an aqueous system at temperatures up to 50° C.
13. A derivatized potato starch comprising the potato starch of claim 1 , wherein the derivatized potato starch is a starch ether, crosslinked starch, oxidized starch, or starch ester.
14. A potato starch comprising an amylose content, as measured by the method of Hovenkamp-Hermelink et al., of less than 10% by weight, a phosphate content in the C6 position is between 35 and 100 nmol of phosphate per milligram of starch (dry weight), a total phosphate content to phosphate content in the C6 position ratio of 1.20-1.50, and a proportion of side chains having a DP of from 12 to 19 which is increased to 125%-200% as compared with that in potato starch from corresponding wild-type potato plants.
15. The potato starch of claim 14 , wherein said phosphate content in the C6 position is between 40 and 85 nmol of phosphate per milligram of starch (dry weight).
16. The potato starch of claim 14 , wherein said phosphate content in the C6 position is between 45 and 70 nmol of phosphate per milligram of starch (dry weight).
17. The potato starch of claim 14 , wherein said amylose content, as measured by the method of Hovenkamp-Hermelink et al., is less than 5% by weight.
18. The potato starch of claim 14 , wherein said amylose content, as measured by the method of Hovenkamp-Hermelink et al. is less than 3% by weight.
19. The potato starch of claim 14 , wherein the proportion of side chains having a DP of from 12 to 19 is increased to 130%-180% as compared with potato starch from corresponding wild-type potato plants.
20. The potato starch of claim 14 , wherein said phosphate content in the C6 position is between 50 and 65 nmol of phosphate per milligram of starch (dry weight).
21. A potato starch isolated from a potato plant cell which exhibits an activity
(a) of one or more SSIII proteins which occur endogenously in the plant cell;
(b) of one or more BEI proteins which occur endogenously in the plant cell;
(c) of one or more GBSSI proteins which occur endogenously in the plant cell; and
(d) of one or more proteins which occur endogenously in the plant cell and which exhibit an at least 90% identity with the amino acid sequence of SEQ ID NO: 12 or SEQ ID NO: 14,
which is reduced as compared with that of corresponding wild-type potato plant cells, and
wherein said starch has a proportion of side chains having a degree of polymerization (DP) of from 12 to 19 which is increased to 125%-200% as compared with potato starch from corresponding wild-type potato plants.
22. The starch of claim 21 , wherein said starch is isolated from a potato plant cell comprising
(a) a first foreign nucleic acid molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(b) a second foreign nucleic acid molecule that reduces the expression of at least one endogenous gene encoding a BEI protein; and
(c) a third foreign nucleic acid molecule that reduces the expression of at least one endogenous gene encoding a GBSSI protein; and
(d) a fourth foreign nucleic acid molecule that reduces the expression of at least one nucleic acid molecule that has an identity of at least 90% with SEQ ID NO: 11 or SEQ ID NO: 13.
23. The starch of claim 22 , wherein
(a) said first foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO: 1 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 1
(b) said second foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO: 4 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 4;
(c) said third foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO 6 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 6; and
(d) said fourth foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO: 11 or 13 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 11 or 13.
24. The starch of claim 22 , wherein
(a) said first foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(b) said second foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein;
(c) said third foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a GBSSI protein;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a GBSSI protein; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a GBSSI protein; and
(d) said fourth foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14.
25. The starch of claim 24 , wherein
(a) said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(b) said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein;
(c) said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a GBSSI protein; and
(d) said fourth foreign nucleic acid molecule is a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14.
26. A method of manufacturing a potato starch comprising extracting starch from a potato plant cell, wherein said plant cell exhibits an activity
(a) of one or more SSIII proteins which occur endogenously in the plant;
(b) of one or more BEI proteins which occur endogenously in the plant;
(c) of one or more GBSSI proteins which occur endogenously in the plant; and
(d) of one or more proteins which occur endogenously in the plant and which exhibit an at least 90% identity with the amino acid sequence of SEQ ID NO: 12 or SEQ ID NO: 14,
which is reduced as compared with that of corresponding wild-type potato plant cells, and
wherein said starch has a proportion of side chains having a degree of polymerization (DP) of from 12 to 19 which is increased to 125%-200% as compared with potato starch from corresponding wild-type potato plants.
27. The method of claim 26 , wherein said plant cell comprises
(a) a first foreign nucleic acid molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(b) a second foreign nucleic acid molecule that reduces the expression of at least one endogenous gene encoding a BEI protein; and
(c) a third foreign nucleic acid molecule that reduces the expression of at least one endogenous gene encoding a GBSSI protein; and
(d) a fourth foreign nucleic acid molecule that reduces the expression of at least one nucleic acid molecule that has an identity of at least 90% with SEQ ID NO: 11 or SEQ ID NO: 13.
28. The method of claim 27 , wherein
(a) said first foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO: 1 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 1
(b) said second foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO: 4 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 4;
(c) said third foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO 6 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 6; and
(d) said fourth foreign nucleic acid molecule is
(i) a nucleic acid molecule, which comprises the nucleotide sequence of SEQ ID NO: 11 or 13 or a complementary sequence thereof; or
(ii) a nucleic acid molecule, which has an identity of at least 95% with nucleic acid sequence with SEQ ID NO: 11 or 13.
29. The method of claim 27 , wherein
(a) said first foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a SSIII protein; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(b) said second foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a BEI protein; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a BEI protein;
(c) said third foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a GBSSI protein;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding a GBSSI protein; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding a GBSSI protein; and
(d) said fourth foreign nucleic acid molecule is
(i) a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14;
(ii) a DNA molecule which, via a co-suppression effect, reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14; or
(iii) a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14.
30. The method of claim 29 , wherein
(a) said first foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a SSIII protein;
(b) said second foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a BEI protein;
(c) said third foreign nucleic acid molecule is a DNA molecule encoding at least one antisense RNA that reduces the expression of at least one endogenous gene encoding a GBSSI protein; and
(d) said fourth foreign nucleic acid molecule is a DNA molecule that simultaneously encodes at least one antisense RNA and at least one sense RNA, where said antisense RNA and said sense RNA form a double-stranded RNA molecule that reduces the expression of at least one endogenous gene encoding SEQ ID NO: 12 or 14.