Immunochemical determination method and determination reagent for cytochrome c
An immunochemical method for the accurate measurement of the quantity of cytochrome c in a body fluid, in particular, blood, and a kit for the measurement. The quantity of cytochrome c can be measured accurately without being affected by any interfering substrate by reacting an antibody with cytochrome c in a buffer solution in an acidic range.
1. A method for immunochemically measuring cytochrome c in a body fluid, which comprises reacting an antibody and cytochrome c in an acidic buffer solution; and measuring the binding of the antibody and cytochrome c to determine the amount of cytochrome c in the body fluid.
2. The method according to claim 1 , wherein the acidic buffer solution has a pH of 3.5 to 5.0.
3. The method according to claim 1 , wherein the body fluid is blood.
4. A kit for immunochemically measuring cytochrome c in a body fluid, comprising an antibody that reacts with cytochrome c; and an acidic buffer solution suitable for reacting the antibody and cytochrome c.
5. The kit according to claim 4 , wherein the acidic buffer has a pH of 3.5 to 5.0.
6. The kit according to claim 4 , wherein the antibody is immobilized or labeled.
7. The method according to claim 2 , wherein the body fluid is blood.
8. The kit according to claim 5 , wherein the antibody is immobilized or labeled.
9. The method according to claim 1 , wherein the antibody is immobilized or labeled.
10. The method according to claim 1 , wherein the acidic buffer has a pH of 3.5 to 4.5.
11. The kid according to claim 4 , wherein the acidic buffer has a pH of 3.5 to 4.5.