Medicament, particularly an anti-cancer medicament, for treatment using immunotherapy, particularly autologous
The invention relates to a medicament characterized in that it comprises at least one solid biocompatible support, preferably in powder form, on which at least one active substance, preferably selected among biological materials and/or biological molecules, is adsorbed or on which it is to be adsorbed, preferably without requiring a coupling agent. Said solid biocompatible support is capable of purifying the active substance. The invention relates to also relates to a method for preparing a medicament of the aforementioned type, this method essentially consisting of placing at least one solid biocompatible support, preferably in powder form, in contact with at least one active substance, preferably selected among biological materials and/or biological molecules, in such a manner that the active substance is reversibly adsorbed and without denaturing on the support.
1. A method for preparing an antitumor vaccine, comprising:
a. obtaining tumor tissue from a patient;
b. grinding the tumor tissue;
c. solubilizing or suspending cytoplasmic tumor proteins of the ground tumor tissue to form an extract;
d. centrifuging the extract to yield a membrane fraction and a supernatant containing the cytoplasmic tumor proteins;
e. combining the supernatant with powdered ceramic hydroxyapatite to form loaded hydroxyapatite; and
f. washing the loaded hydroxyapatite with a buffer solution to recover a composition comprising cytoplasmic tumor proteins adsorbed onto powdered ceramic hydroxyapatite
wherein said powdered ceramic hydroxyapatite consists of a powder of spherically shaped particles of hydroxyapatite having the following characteristics:
a particle size between 1-30 μm, and
having been exposed to a sintering temperature of 600° C.
2. A composition comprising cytoplasmic tumor proteins adsorbed on powdered ceramic hydroxyapatite, wherein the powdered ceramic hydroxyapatite is a powder of spherically shaped particles of hydroxyapatite having the following characteristics:
a particle size between 1-30 μm, and
having been exposed to a sintering temperature of 600° C.
3. A composition according to claim 2 , obtained by the following steps:
a. obtaining tumor tissue from a patient;
b. grinding the tumor tissue;
c. solubilizing or suspending cytoplasmic tumor proteins of the ground tumor tissue to form an extract;
d. centrifuging the extract to yield a membrane fraction and a supernatant containing the cytoplasmic tumor proteins;
e. combining the supernatant with powdered ceramic hydroxyapatite to form loaded hydroxyapatite; and
f. washing the loaded hydroxyapatite with a buffer solution to recover a composition comprising cytoplasmic tumor proteins adsorbed onto powdered ceramic hydroxyapatite; said powdered ceramic hydroxyapatite consisting of a powder of spherically shaped particles of hydroxyapatite having the following characteristics:
a particle size between 1-30 μm, and having been exposed to a sintering temperature of 600° C.
4. A method for preparing an antitumor vaccine, comprising:
a. obtaining tumor tissue from a patient;
b. grinding the tumor tissue;
c. solubilizing or suspending cytoplasmic tumor proteins of the ground tumor tissue to form an extract;
d. centrifuging the extract to yield a membrane fraction and a supernatant containing the cytoplasmic tumor proteins;
e. combining the supernatant with powdered ceramic hydroxyapatite to form loaded hydroxyapatite; and
f. washing the loaded hydroxyapatite with a buffer solution to recover a composition comprising cytoplasmic tumor proteins adsorbed onto powdered ceramic hydroxyapatite; said powdered ceramic hydroxyapatite consisting of a powder of spherically shaped particles of hydroxyapatite having the following characteristics: a particle size between 1-30 μm, and having been exposed to a sintering temperature of 600° C.
5. A composition cytoplasmic tumor proteins adsorbed on powdered ceramic hydroxyapatite obtained by the method of claim 1 .
6. An antitumor vaccine obtained by the method of claim 4 .