FEED OR FOOD PRODUCTS COMPRISING FUNGAL MATERIAL
The present invention relates to feed and food compositions comprising material obtained by fermenting fungi of the Basidiomycetes family in a liquid medium. Interestingly, Basidiomycetes grown in a liquid culture produce compounds which, when used in feed or food compositions enhance survival and/or support growth of normal, healthy animals. Furthermore, the compounds may modulate the microbial population in the digestive tract after oral intake.
1 . A feed or food product comprising extracellular material derived from a liquid culture of a fungus of the class of Basidiomycetes, wherein said extracellular material comprises a survival enhancing agent and is obtainable by a method comprising the steps of:
i) fermenting a fungus of the class of Basidiomycetes in a liquid culture,
ii) isolating the extracellular material by removal of the biomass, thereby obtaining an extracellular liquid,
iii) optionally isolating an extracellular composition comprising the survival enhancing agent from the extracellular liquid.
2 . The feed or food product according to claim 1 , wherein said survival enhancing agent comprises polysaccharides.
3 . The feed or food product according to claim 1 , wherein the survival enhancing agent comprises polypeptides.
4 . The feed or food product according to claim 2 , wherein at least part of the polysaccharides have a molecular weight of at least 30,000 Da.
5 . The feed product according to claim 1 , wherein the feed product is an aquatic animal feed product.
6 . The feed product according to claim 1 , wherein the feed product is a crustacean feed product.
7 . The feed product according to claim 1 , wherein said extracellular material is comprised within a living microorganism to which the extracellular material has been fed, or administered in another way.
8 . The feed product according to claim 1 , wherein said extracellular material is comprised within a dead organism to which the extracellular material has been fed or administered in another way.
9 . The feed product according to claim 7 , wherein said organisms are artemia, rotifer and/or calanus.
10 . The feed product according to claim 1 , wherein the feed product is a farm animal feed product.
11 . The feed product according to claim 1 , wherein the feed product is an artemia or rotifer feed product.
12 . The feed product of claim 11 , wherein the feed product is in the form of an emulsion comprising an organic phase and a water soluble phase.
13 . The food or feed product of claim 1 , wherein the fungus is a species of Tricholomatales.
14 . The food or feed product of claim 1 , wherein the fungus is a species of Agaricales.
15 . The food or feed product of claim 1 , wherein the fungus is a species of Marasmiaceae.
16 . The food or feed product of claim 1 , wherein the fungus is a species of Lentinus ( Lentinula ).
17 . The food or feed product of claim 1 , wherein the fungus is Lentinus ( Lentinula ) edodes.
18 . The food or feed product of claim 1 , wherein the culturing in step i) is performed at 25° C., pH 3 to 7 for 50 to 300 hours.
19 . The food or feed product of claim 1 , wherein the liquid culture comprises malt extract and optionally glucose, yeast extract and peptone.
20 . The food or feed product of claim 1 , where step ii) comprises centrifugation and/or filtration of the liquid culture.
21 . The food or feed product of claim 1 , wherein isolation of the extracellular composition comprising the survival enhancing agent, comprises one or more of the following steps:
size fractionation and recovery of molecules above 30,000 kD
ethanol precipitation and recovery of the pellet
drying, such as freeze drying or lyophilisation
spraying and drying
removal of low molecular weight material
removal of oxidants
isolation of polysaccharides
production of an emulsion comprising of an organic phase and a watersoluble phase.
22 . The food or feed product according to claim 21 , wherein size fractionation is a method involving ultracentrifugation, ultrafiltration, microfiltration or gelfiltration.
23 . The food or feed product according to claim 1 , wherein only fungal mycelium of said Basidiomycete is fermented.
24 . The food or feed product according to claim 1 , wherein said Basidiomycete is selected from the group consisting of Agaricus bisporus, Cordiceps sinensis, Flammulina velutipes, Ganoderma lucidum, Grifola frondosa, Lentinus edodes, Pleurotus ostreatus, Schizophyllum commune, Sclerotina sclerotium, Trametes ( Coriolus ) versicolor, Tremella fuciformis, Agaricus blazei, Agrocybe aegerita, Agrocybe cylindracea, Albatrellus confluens, Armillariella mellea, Auricularia auricula - judae, Auricularia polytricha, Collybia maracula, Cordiceps militari, Dendropolyporus umbellatus, Fomes fomentarius, Fomes pinicola, Ganoderma applanatum, Ganoderma tsugae, Hericium erinaceus, Hypsizygus marmoreus, Inonotus obliquus, Laetiporus sulphurous, Lenzites betulinus, Leucopaxilllus giganteus, Lyophyllum cinerascens, Omphalina epichysium, Oudemansiella mucida, Panellus serotinus, Piptoporus betulinus, Phellinus linteus, Phellinus pini, Pholiota nameko, Pleurotus citrinopileatus, Pleurotus pulmonarius, Sarcedon asparatus, Trametes suavolens, Volvariella volvacea and Wolfiporia cocos.
25 . The food or feed product according to claim 24 , wherein said Basidiomycete is selected from the group consisting of Agaricus bisporus, Cordiceps sinensis, Flammulina velutipes, Ganoderma lucidum, Grifola frondosa, Lentinus edodes, Pleurotus ostreatus, Schizophyllum commune, Sclerotina sclerotium, Trametes ( Coriolus ) versicolor and Tremella fuciformis.
26 . The food or feed product according to claim 1 , wherein the survival enhancing agent comprises a mixture of polysaccharides comprising the monosaccharides galactose, mannose, and glucose in the ratio 1:0 to 25:1 to 50.
27 . The food or feed product according to claim 1 , wherein the survival enhancing agent comprises at least 10 μg/L soluble polypeptide.
28 . The food or feed product according to claim 1 , wherein said survival enhancing agent comprises in the range of 10 to 1000 μg/L soluble polypeptide.
29 . A method for producing a food or feed product of claim 1 comprising the steps of
i) fermenting a fungus of the class of Basidiomycetes in a liquid culture,
ii) isolating the extracellular material by removal of the biomass,
iii) optionally performing one or more of the steps specified in claim 21 and 22 , and
iv) incorporating the product of step ii) or iii) into a food or feed product.
30 . A method of feeding an animal comprising feeding said animal a feed product as defined in claim 1 .
31 . The method of claim 30 , wherein the feed product is an aquatic or farm animal feed product.
32 . The method according to claim 30 , wherein the method comprises feeding a prey organism the feed product and subsequently feeding said prey organism to an animal.
33 . The method according to claim 32 , wherein the prey organism is zoo plankton and the animal is a marine fish or a shell fish.
34 . A farm animal feed product comprising biomass derived from a liquid culture of a fungus of the class of Basidiomycetes, wherein said biomass comprises a survival enhancing agent and is obtainable by a method comprising the steps of:
i) fermenting a fungus of the class of Basidiomycetes in a liquid culture,
ii) isolating the biomass by removal of the extracellular material
iii) optionally isolating a composition comprising the survival enhancing agent.
35 . An aquatic animal feed product comprising biomass derived from a liquid culture of a fungus of the class of Basidiomycetes, wherein said biomass comprises a survival enhancing agent and is obtainable by a method comprising the steps of:
i) fermenting a fungus of the class of Basidiomycetes in a liquid culture,
ii) isolating the biomass by removal of the extracellular material
iii) optionally isolating a composition comprising the survival enhancing agent.
36 . (canceled)
37 . A food or feed product comprising biomass derived from a liquid culture of a fungus of the class of Basidiomycetes, wherein said biomass comprises a survival enhancing agent and is obtainable by a method comprising the steps of:
i) fermenting a fungus of the class of Basidiomycetes in a liquid culture in the presence of malt extract,
ii) isolating the biomass by removal of the extracellular material
iii) optionally isolating a composition comprising the survival enhancing agent.
38 . The feed product according to claim 34 , wherein the survival enhancing agent comprises polysaccharides.
39 . The feed product according to claim 34 , wherein the survival enhancing agent comprises polypeptides.
40 . The feed product according to claim 34 , wherein at least part of the polysaccharides have a molecular weight of at least 30,000 Da.
41 . The feed or food product according to claim 1 , wherein said product is a functional food suitable for consumption by humans beings.
42 . The functional food according to claim 41 , wherein said functional food is a dairy product.
43 . The functional food according to claim 41 , wherein said functional food is a bread.
44 . The functional food according to claim 41 , wherein said functional food is a fruit juice-based product.