Compositions of partially deacetylated chitin derivatives
The present invention relates to compositions comprising biologically active chitinous oligomers and their endotoxin purified and partially deacetylated chitin polymer precursors, and their use in pharmaceutical compositions, biomaterial compositions, medical devices, and processes to produce the said oligomers. More specifically the present invention relates to novel compositions and processes to produce such compositions. The compositions include therapeutic hetero polymer and hetero oligomer compositions comprising specific sequences of N-acetyl glucosamine and glucosamine, developed to optimize chemical and structural features which are important for the therapeutic activity of these compositions. In addition, the present invention provides methods to use degree of deacetylation of a partially deacetylated chitin polymer in order to modulate physical and biological parameters in a calcium phosphate composite for bone implant applications.
1. A dual component biomaterial composition comprising a solid component and a liquid component, for in situ crystallization of said composition, for enhancing bone regeneration and haemostasis in the healing of a fractured or severed bone in a mammal, said solid component comprising soluble partially deacetylated chitin with a degree of deacetylation (DD) between 30% and 60% and calcium phosphate,
said liquid component comprising an aqueous acidic medium, and
wherein the partially deacetylated chitin is prepared by a process comprising:
partially deacetylating chitin polymer with alkali treatment, wherein said alkali is cooled prior to mixing with the chitin, the deacetylation reaction maintained at a temperature in the range of 5-60° C.;
neutralizing the partially deacetylated chitin;
removing undissolved particles through sequential filtering steps;
adjusting the solution to a pH above 8, and raising the temperature of the solution to above 30° C.;
precipitating the fully dissolved and purified partially deacetylated chitin; and
recovering and washing said precipitate at a temperature above 50° C.
2. The composition according to claim 1 , wherein the biomaterial composition further comprises at least one component selected from the group consisting of hydroxyapatite, calcium sulphate, sodium tripolyphosphate, alginate, collagen and hyaluronic acid.
3. A composition according to claim 1 , wherein said solid component comprises said partially deacetylated chitin and calcium phosphates, and said liquid component comprises phosphoric acid.
4. A composition according to claim 1 , comprising about 5 wt % of said partially deacetylated chitin.