Methods of identifying compounds for the treatment of sterile inflammation
Described are methods and compositions that inhibit IL-1 signalling for the treatment of acute inflammatory response to cell necrosis, and the attendant collateral tissue damage.
1. A method of identifying a candidate compound useful in the treatment, prevention, or delay of development or progression of a condition associated with sterile inflammation induced by cell death, the method comprising:
providing a sample comprising MyD88;
contacting the sample with one or more test compounds; and
detecting specific binding of a test compound to the MyD88 in the sample;
selecting a test compound that binds specifically to the MyD88;
providing a cell comprising functional MyD88, IL-1 and IL-1 receptor;
contacting the cell with the test compound;
assaying MyD88/IL-1 signalling in the cell in the presence of the test compound;
wherein a test compound that inhibits MyD88/IL-1 signalling in the cell is a candidate compound useful in the treatment, prevention, or delay of development or progression of a condition associated with sterile inflammation induced by cell death.