Method for preserving cells, tissues or organs using a solution comprising alkyldiamine-beta-cyclodextrin
A composition for protecting and/or preserving cells, tissues or organs, includes at least one cyclodextrin, preferably closely combined with an antioxidant. A process for producing this composition and its uses are also disclosed.
1. A method of protecting, preserving and/or maintaining organs, tissues or cells ex vivo, comprising preserving the organs, tissues or cells with an effective amount of a composition comprising 6A-[(n-aminoalkyl)amino]-6A-deoxy-β-cyclodextrin.
2. The method of claim 1 , wherein the composition comprises the cyclodextrin closely combined with an antioxidant.
3. The method of claim 2 , wherein the antioxidant is resveratrol.
4. The method of claim 2 , wherein the antioxidant is curcumine.
5. The method of claim 2 , wherein the composition comprises an antioxidant/cyclodextrin molar ratio of between 1/1.5 and 1/150.
6. The method of claim 5 , wherein the antioxidant is resveratrol.
7. The method of claim 5 , wherein the antioxidant is curcumine.
8. The method of claim 1 , wherein the composition further comprises an impermeable agent and a buffer.
9. The method of claim 1 , wherein the organs are preserved in cold ischemia.
10. The method according to claim 1 , wherein the composition comprises 6A-[(3-aminopropyl)amino]-6A-deoxy-β-cyclodextrin, or 6A-[(2-aminoethyl)amino]-6A-deoxy-β-cyclodextrin, or a combination thereof.