Preservation of bioactive materials by spray drying
This invention provides methods and compositions to preserve bioactive materials in a matrix of powder particles. Methods provide high-pressure gas spraying and/or near supercritical spraying of formulations followed by drying in a stream of conditioned gas to form stable powder particles containing bioactive materials.
1. A mixture of a high pressure gas with a suspension or solution, the mixture comprising: a high pressure gas, a bioactive material, a polyol, a polymer, and a surfactant, wherein the mixture forms stable powder particles on spraying and drying.
2. The mixture of claim 1 , wherein the bioactive material is selected from the group consisting of proteins, peptides, nucleic acids, bacteria, cells, antibodies, enzymes, serums, vaccines, liposomes, and viruses.
3. The mixture of claim 2 , wherein the viruses are selected from the list consisting of influenza virus, parainfluenza virus, respiratory syncytial virus, herpes simplex virus, cytomegalo virus, SARS virus, corona virus family members, human metapneumovirus, and Epstein-Bar virus.
4. The mixture of claim 1 , wherein the polyol is sucrose present in an amount of about 10 weight percent of the suspension or solution.
5. The mixture of claim 1 , wherein the bioactive material comprises influenza virus, the polyol comprises sucrose, the polymer comprises HES, and the surfactant comprises a block copolymer of polyethylene and polypropylene glycol.
6. An article of manufacture comprising a container containing dried powder particles prepared by spray drying a mixture of high pressure gas with a suspension or solution of bioactive material, a polyol, a polymer, and a surfactant.