Electrophoresis unit and electrophoretic analysis method
View Patent ↗The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased. An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.
1. An electrophoresis unit having a plurality of electrophoretic channels, an excitation optical system for irradiating a sample separated by electrophoresis on said electrophoretic channels with an excitation light, a detector for detecting fluorescence emitted from said sample, and a data viewing area for displaying fluorescence intensity of said sample, wherein:
a data binning area of said detector is controlled during electrophoretic analysis, and
a viewing scale of the fluorescence intensity changes on the data viewing area to avoid a saturation state when the fluorescence intensity is saturated or before the fluorescence intensity is saturated.
2. The electrophoresis unit according to claim 1 , further comprising an LED light source for emitting the excitation light.
3. The electrophoresis unit according to claim 1 , wherein a limit range (maximum analysis value-minimum analysis value) of the fluorescence intensity can be displayed by recording the fluorescence intensity for each electrophoretic channel.
4. The electrophoresis unit according to claim 1 , wherein spectral calibration data processing is performed for a plurality of data binning areas when spectral calibration data is created.
5. The electrophoresis unit according to claim 1 , wherein sampling data for which actual fluorescence intensity could not be measured due to saturation is interpolated by using an approximate function based on data before/after the saturation.
6. An electrophoretic analysis method comprising:
preparing a plurality of electrophoretic channels;
separating a sample by electrophoresis on the electrophoretic channels;
irradiating the sample separated by electrophoresis with an excitation light by means of an excitation optical system;
detecting fluorescence emitted from the sample by a detector; and
displaying fluorescence intensity of the sample on a data viewing area, wherein:
a data binning area of said detector is controlled, and
a viewing scale of the fluorescence intensity changes on the data viewing area to avoid a saturation state when the fluorescence intensity is saturated or before the fluorescence intensity is saturated.