DNA-transfection system for the generation of infectious influenza virus
The present invention is based on the development of a dual promoter system (preferably a RNA pol I-pol II system) for the efficient intracellular synthesis of viral RNA. The resultant minimal plasmid-based system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. The viral product of the system is produced when the plasmids of the system are introduced into a suitable host cell. One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. The reassortant viruses generated by cotransfection of plasmids may comprise genes encoding the surface glycoproteins hemagglutinin and neuraminidase from an influenza virus currently infecting the population and the internal genes from an attenuated influenza virus. An advantageous property of the present invention is its versatility; the system may be quickly and easily adapted to synthesize an attenuated version of any RNA virus. Attenuated or inactivated RNA viruses produced by the present invention may be administered to a patient in need of vaccination by any of several routes including intranasally or intramuscularly.
1 - 41 . (canceled)
42 . A method of preparing an infectious negative strand RNA virus-specific vaccine, which method comprises purifying a negative strand RNA virus virion produced by culturing a host cell, wherein a set of plasmid is introduced into said host cell, wherein each plasmid of said set comprises one viral genomic segment of said negative strand RNA virus, and wherein the viral cDNA corresponding to the viral genomic segment is inserted between an RNA polymerase I (pol I) promoter and a regulatory element, which results in expression of vRNA or cRNA with an exact 3′ end, which are in turn inserted between an RNA polymerase II (pol II) promoter and a polyadenylation signal, which results in expression of viral mRNA and a corresponding viral protein, wherein the expression of vRNAs or cRNAs and viral proteins from said set of plasmids results in an infectious negative strand RNA virus virion.
43 . The method of claim 42 , wherein the negative strand RNA virus is attenuated.
44 . The method of claim 42 , wherein the negative strand RNA virus is an influenza virus.
45 . A method of claim 42 , which further comprises inactivating the virion.
46 . The method of claim 45 , wherein said virion is inactivated by a treatment selected from the group consisting of formaldehyde, beta-propiolactone, ether, ether with a detergent, cetyl trimethyl ammonium bromide (CTAB) and Triton N101, and sodium deoxycholate and tri(n-butyl) phosphate.
47 . The method of claim 42 , which further comprises a clarification of allantoic fluid from virus.
48 . The method of claim 47 , wherein said virion is inactivated prior to the clarification of allantoic fluid from virus.
49 . The method of claim 47 , wherein the virion is inactivated after the clarification of an allantoic fluid.
50 . The method of claim 42 , which further comprises assessing the potency of said virion by a single radial immunodiffusion (SRI) test.
51 . The method of claim 42 , wherein said vaccine further comprises a carrier.
52 . The method of claim 42 , wherein said vaccine further comprises an adjuvant.
53 . The method of claim 52 , wherein said adjuvant is selected from the group consisting of complete Freund's adjuvant, incomplete Freund's adjuvant, saponin, mineral gels, aluminum hydroxide, surface active substances, lysolecithin, pluronic polyols, polyanions, peptides, oil, hydrocarbon emulsions, BCG (bacille Calmette-Guerin), Corynebacterium parvum and QS-21.
54 . A method for vaccinating a subject against an infectious segmented negative strand RNA virus infection, which method comprises administering to a subject in need thereof a protective dose of a vaccine composition comprising an infectious segmented negative strand RNA virus, wherein said vaccine is produced by a minimum plasmid-based system comprising a set of plasmids, which results in assembly of an infectious segmented negative RNA virus upon introduction in a host cell, wherein each plasmid of said set comprises one viral genomic segment, and wherein the viral cDNA corresponding to the viral genomic segment is inserted between an RNA polymerase I (pol I) promoter and a regulatory element, which results in expression of vRNA or cRNA with an exact 3′ end, which are in turn inserted between an RNA polymerase II (pol II) promoter and a polyadenylation signal, which results in expression of viral mRNA and a corresponding viral protein, wherein the expression of vRNAs or cRNAs and viral proteins from said set of plasmids results in an infectious segmented negative strand RNA virus.
55 . The method of claim 54 , wherein said administering is via intramuscular injection.
56 . The method of claim 54 , wherein said administering is via intranasal route.
57 . The method of claim 54 , wherein said subject in need thereof is an animal.
58 . The method of claim 57 , wherein said animal is a human.
59 . The method of claim 54 , wherein said vaccine composition induces an immune response.
60 . The method of 59 , wherein said immune response is a local immunity, a mucosal immunity, a cell mediated immunity, or a humoral immunity, or a combination of one or more immunities thereof.
61 . The method of claim 54 , wherein said vaccine composition prevents or treats a disease or disorder associated with an infection by said infectious segmented negative strand RNA virus when administered to a subject in need thereof.
62 . The method of claim 59 , wherein said administering is via intramuscular injection.
63 . The method of claim 59 , wherein said administering is via intranasal route.
64 . The method of claim 59 , wherein said subject in need thereof is an animal.
65 . The method of claim 64 , wherein said animal is a human.