Methods and compositions for protein labeling using lipoic acid ligases
The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants.
1. A method for labeling a target protein, the method comprising
contacting a fusion protein with a lipoic acid analog, and
allowing sufficient time for the lipoic acid analog to be conjugated to the fusion protein via an acceptor polypeptide, in the presence of a lipoic acid ligase polypeptide that comprises an amino acid sequence of a wild-type lipoic acid ligase or a mutant thereof, the mutant being at least 90% identical to SEQ ID NO:11 and having lipoic acid ligase activity;
wherein the fusion protein is a fusion of the target protein and the acceptor polypeptide, which comprises an amino acid sequence at least 90% identical to SEQ ID NO: 3, 5, 6, 8, or 10, and
wherein the lipoic acid analog comprises an alkyl azide, an alkyne, an alkyl halide, an aryl azide photoaffinity probe, a diazirine photoaffinity probe, a benzophenone photoaffinity probe, or a fluorophore.
2. The method of claim 1 , wherein the fluorophore is a coumarin fluorophore.
3. The method of claim 1 , wherein the lipoic acid analog is conjugated to a detectable label.
4. The method of claim 3 , wherein the directly detectable label is coumarin, fluorescein, an aryl azide, a diazirine, a benzophenone, a resorufin, a xanthene-type fluorophore, a chloroalkane, a metal-binding ligand, or a derivative thereof.
5. The method of claim 1 , wherein the lipoic acid analog is labeled with an indirectly detectable label.
6. The method of claim 5 , wherein the indirectly detectable label is an enzyme.
7. The method of claim 1 , wherein the lipoic acid analog is labeled with a membrane impermeant label.
8. The method of claim 1 , wherein the lipoic acid analog is labeled with a cyclooctyne conjugate.
9. The method of claim 1 , wherein the acceptor polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO: 3, 5, 6, 8, or 10.
10. The method of claim 9 , wherein the acceptor polypeptide comprises the amino acid sequence of SEQ ID NO: 3, 5, 6, 8, or 10.
11. The method of claim 9 , wherein the acceptor polypeptide comprises the amino acid sequence of SEQ ID NO: 10.
12. The method of claim 1 , wherein the lipoic acid ligase polypeptide comprises an amino acid sequence of a mutant of a wild-type lipoic acid ligase, the mutant being at least 95% identical to SEQ ID NO:11.
13. The method of claim 1 , wherein the lipoic acid ligase polypeptide comprises the amino acid sequence of a wild-type E. coli LplA.
14. The method of claim 1 , wherein the lipoic acid ligase polypeptide comprises an amino acid of a mutant of a wild-type lipoic acid ligase, the mutant, as compared to SEQ ID NO:11, including a substitution at one or more of residues corresponding to residue(s) 16, 17, 19, 20, 21, 37, 37+71, 37+20, 37+35, 35, 41, 70, 71, 72, 79, 85, 87, 140, 147, and 149 of SEQ ID NO :11.
15. The method of claim 1 , wherein the lipoic acid ligase polypeptide comprises an amino acid sequence of a mutant of a wild-type lipoic acid ligase, the mutant, as compared to SEQ ID NO:11, including one or more of the amino acid substitution(s) corresponding to N16A, L17A, V19A, E20A, E21A, W37A, W37G, W37S, W37V, W37A+S71A, W37A+E20A, W37L, W37I, W37T, W37N, W37V+E20G, W37V+F35A, W37V+E20A, F35A, N41A, R70A, S71A, S72A, H79A, C85A, T87A, R140A, F147A, H149A, or H149V of SEQ ID NO :11.
16. The method of claim 13 , wherein the lipoic acid ligase polypeptide comprises the amino acid sequence of SEQ ID NO:11.
17. The method of claim 12 , wherein the lipoic acid ligase polypeptide comprises an amino acid sequence of a mutant of a wild-type lipoic acid, the mutant being at least 99% identical to SEQ ID NO: 11.
18. The method of claim 1 , wherein the lipoic acid ligase polypeptide comprises an amino acid sequence of a homolog of an E. coli lipoic acid ligase.
19. The method of claim 1 , wherein the lipoic acid analog has the structure of one of:
20. The method of claim 12 , wherein the mutant, as compared to SEQ ID NO:11, includes a substitution at one or more of residues corresponding to residue(s) 16, 17, 19, 20, 21, 37, 37+71, 37+20, 37+35, 35, 41, 70, 71, 72, 79, 85, 87, 140, 147, and 149 of SEQ ID NO :11.