Electrophoretic apparatus
View Patent ↗An electrophoretic apparatus that allows bubbles to be readily removed out of an electrophoretic passage. The passage for electrophoretic medium, at a connecting section where capillaries filled with electrophoretic medium are connected with a pumping mechanism for filling the electrophoretic medium, is arranged such that the side of the pumping mechanism is disposed below the side of the capillaries, so that the electrophoretic medium flows from down to up at the connecting section when filling the electrophoretic medium into the capillaries. Preferably, the passage between the capillary array and the buffer solution is controlled by using a rotary-type valve having high withstand pressure to simplify the passage structure. The dead volume of the passage can be reduced and the valuable electrophoretic medium can be efficiently used. The amount of used electrophoretic medium required for the removal of the bubble can be also reduced.
1. A capillary electrophoretic apparatus, comprising:
one or more capillaries in which electrophoretic medium is filled;
a first connecting passage that can be connected with the one or more capillaries;
a pump that communicates with the first connecting passage and that can fill the electrophoretic medium into the one or more capillaries;
a second connecting passage that communicates with the connecting passage,
a buffer container that communicates with the second connecting passage and stores buffer solution into which an electrode for applying voltage to the electrophoretic medium is soaked; and
a valve provided in the second passage,
wherein:
an end face of the one or more capillaries connected with the first connecting passage faces downward and is positioned below a part where the second connecting passage communicates with the first connecting passage.
2. The electrophoretic apparatus according to claim 1 , wherein the valve has a rotor, stators for sandwiching the rotor, and a push screw for pressing down the rotor and the stators.
3. The electrophoretic apparatus according to claim 1 , wherein the valve is a rotary-type valve.
4. A capillary electrophoretic apparatus, comprising:
one or more capillaries in which electrophoretic medium is filled;
a first connecting passage that can be connected with the one or more capillaries;
a pump that communicates with the first connecting passage and that can fill the electrophoretic medium into the one or more capillaries;
a second connecting passage that communicates with the connecting passage,
a buffer container that communicates with the second connecting passage and stores buffer solution into which an electrode for applying voltage to the electrophoretic medium is soaked,
wherein:
an end face of the one or more capillaries connected with the first connecting passage faces downward and is positioned below a part where the second passage communicates with the first connecting passage,
the second connecting passage is bent so as to have at least one apex that is positioned above the level of the buffer solution, and
a valve for opening/closing the second connecting passage is provided at position between the apex and the level of the buffer solution in the second connecting passage.
5. The electrophoretic apparatus according to claim 4 , wherein the valve is a rotary-type valve.