Transcription factors in neuronal dendrites-dendritic protein synthesis and cell death
View Patent ↗The present invention relates to methods of altering the competence of a dendrite and/or the viability of a neuron by modulating the level of Elk-1 in a dendrite. The present invention also provides methods of altering the ATP levels in a neuron, methods of isolating at least one protein of a mitochondrial permeability transition pore complex, methods of introducing an RNA into a neuron, methods of translating an RNA in a dendrite, methods of monitoring risk of neurodegeneration of a neuron, and methods of treatment for neurodegenerative diseases.
1. A method of increasing competence of a dendrite in need of increased competence, the method comprising decreasing the level of Elk-1 in the dendrite in need of increased competence, wherein decreasing the level of Elk-1 in the dendrite in need of increased competence comprises locally administering an inhibitor of Elk-1 expression to the dendrite in need of increased competence, wherein the inhibitor of Elk-1 expression is an Elk-1 siRNA, and further wherein decreasing the level of Elk-1 in the dendrite in need of increased competence increases competence of the dendrite.
2. The method of claim 1 , wherein increasing the competence of a dendrite attached to a neuron leads to increased viability of the neuron.
3. A method of increasing viability of a neuron in need of prolonged viability, the method comprising decreasing the level of Elk-1 in a dendrite attached to the neuron in need of prolonged viability, wherein decreasing the level of Elk-1 in the dendrite attached to the neuron in need of prolonged viability comprises locally administering an inhibitor of Elk-1 expression to the dendrite attached to the neuron in need of prolonged viability, wherein the inhibitor of Elk-1 expression is an Elk-1 siRNA, and further wherein decreasing the level of Elk-1 in the dendrite attached to the neuron in need of prolonged viability increases viability of the neuron.