Plant transformed with hardy (HRD) gene having enhanced drought tolerance
Transgenic plants with novel phenotypes, especially plants with enhanced drought and pathogen resistance. Provided are transgenic crop plants having integrated in their genome a chimeric gene, wherein the chimeric gene includes a transcription regulatory sequence active in plant cells operably linked to a nucleic acid sequence encoding a protein having the sequence of SEQ ID NO: 3 or a protein at least 70% identical to SEQ ID NO: 3 or an ortholog or a functional fragment thereof. In addition to enhanced drought tolerance the transgenic plants may show enhanced disease resistance and enhanced root structure. Also, a method for generating a transgenic plant by insertion of the chimeric gene.
1. A method for the generation of a transgenic plant having at least one phenotype selected from the group consisting of enhanced disease resistance and enhanced root structure, the method comprising the steps of:
(a) introducing into cells of plants a chimeric gene comprising a transgenic regulatory sequence active in plant cells operably linked to a nucleic acid sequence encoding a protein comprising SEQ ID NO: 3;
(b) growing the plant for a period of time and screening the plants for the at least one phenotype to recover a plant containing the chimeric gene as a stable genomic insert, said screening comprising (i) growing the plants in the presence of a pathogen or (ii) examining the root structure of the plants to ascertain the presence of more longitudinal secondary and tertiary roots than in control plants; and, optionally,
(c) obtaining seed from the recovered plant that contain the chimeric gene as a stable genomic insert and that have the at least one phenotype.
2. The method according to claim 1 , wherein said transcription regulatory sequence is selected from the group consisting of: a constitutive promoter, an inducible promoter, a tissue-specific promoter and a developmentally regulated promoter.
3. The method according to claim 1 , wherein said transcription regulatory sequence is a stress inducible promoter.
4. The method according to claim 2 , wherein said transcription regulatory sequence is a tissue-specific promoter.
5. The method according claim 1 , wherein the encoding nucleic acid sequence comprises SEQ ID NO: 1.
6. The method according to claim 1 , wherein the screening comprises growing the plants in the presence of a pathogen.
7. The method according to claim 6 , wherein the pathogen is a fungus.
8. The method according to claim 7 , wherein the fungus is Verticillium.
9. The method according to claim 1 , wherein the screening comprises examining a root structure of the plants to ascertain the presence of more longitudinal secondary and tertiary roots than in control plants.
10. The method according to claim 1 comprising the step of obtaining seed from the recovered plant that contain the chimeric gene as a stable genomic insert and that have the at least one phenotype.
11. The method according to claim 6 comprising the step of obtaining seed from the recovered plant that contain the chimeric gene as a stable genomic insert and that have the at least one phenotype.
12. The method according to claim 9 comprising the step of obtaining seed from the recovered plant that contain the chimeric gene as a stable genomic insert and that have the at least one phenotype.